Characterization of Pectobacterium brasiliense strains from potato and vegetables in Israel,Plant Pathology

当前位置： X-MOL 学术 › Plant Pathol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Characterization of Pectobacterium brasiliense strains from potato and vegetables in Israel
Plant Pathology ( IF 2.3 ) Pub Date : 2021-08-21 , DOI: 10.1111/ppa.13454
Leah Tsror (Lahkim) ₁ , Valerie Hélias ₂ , Sara Mordechai‐Lebiush ₁ , Orly Erlich ₁ , Marina Hazanovsky ₁ , Laura Chalupowicz ₃ , Michal Reuven ₃ , Orit Dror ₃ , Lea Valinsky ₄ , Angelique Laurent ₂ , Shulamit Manulis‐Sasson ₃

Affiliation

Pectobacterium brasiliense (Pbr) infects a wide range of crops worldwide, causing potato blackleg and soft rot and vegetable soft rots. This study aimed to characterize the genetic diversity and virulence variability among 68 Pbr strains isolated from either symptomless potato progeny tubers, diseased potato plants, ware potatoes wash water, or vegetables grown in Israel, as well as strains isolated from symptomless seed tubers grown in Europe, or diseased potato plants grown in France. The collection was typed using PCR and TaqMan real-time PCR analyses, dnaX sequence analysis, pulsed-field gel electrophoresis (PFGE), and pectolytic activity. dnaX phylogeny grouped almost all strains in a common genetic clade related to Pbr, which was distinct from the other Pectobacterium species. PFGE analysis identified two main clusters, including one major group of 47 strains with 95%–100% similarity. Maceration assays on two potato cultivars showed significant differences between strains but with no correlations with the source of the strains nor the status of the host (with/without symptoms). Molecular (dnaX sequences and PFGE profiles) and phenotypic analyses (tuber maceration tests) showed that the tested Pbr strains are not a homogeneous group. Analysis of the tested Pbr strains isolated from potato and vegetables grown in fields with a history of potato cultivation suggests that seed tubers imported from Europe may be the main source for Pbr in Israel. To the best of our knowledge, this is the first study that describes biodiversity and population structure of P. brasiliense isolated from potato and vegetables under hot climate conditions.

中文翻译：

以色列马铃薯和蔬菜中巴西果杆菌菌株的特征

Pectobacterium brasiliense ( Pbr ) 感染世界范围内的多种作物，导致马铃薯黑腿病和软腐病以及蔬菜软腐病。本研究旨在表征从无症状马铃薯后代块茎、患病马铃薯植株、洁具马铃薯洗涤水或以色列种植的蔬菜中分离的 68 种 Pbr 菌株的遗传多样性和毒力变异性，以及从欧洲种植的无症状种子块茎中分离的菌株的遗传多样性和毒力变异性。，或在法国种植的患病马铃薯植株。使用 PCR 和 TaqMan 实时 PCR 分析、dnaX序列分析、脉冲场凝胶电泳 (PFGE) 和果胶活性对集合进行分型。dnaX系统发育将几乎所有菌株归为一个与 Pbr 相关的共同遗传进化枝，这与其他菌株不同。果杆菌属。PFGE 分析确定了两个主要集群，包括一个主要的 47 个菌株组，具有 95%–100% 的相似性。对两个马铃薯品种的浸渍分析显示菌株之间存在显着差异，但与菌株来源和宿主状态（有/无症状）没有相关性。分子 ( dnaX序列和 PFGE 谱）和表型分析（块茎浸渍试验）表明，所测试的 Pbr 菌株不是同质组。对从有马铃薯种植历史的田间种植的马铃薯和蔬菜中分离出的测试 Pbr 菌株的分析表明，从欧洲进口的种子块茎可能是以色列 Pbr 的主要来源。据我们所知，这是第一项描述在炎热气候条件下从马铃薯和蔬菜中分离出来的巴西假单胞菌的生物多样性和种群结构的研究。

更新日期：2021-08-21

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南