The crude polysaccharides (NLCEP) were extracted from Notarchus leachii freeri eggs strings by the salt extraction method. The extraction conditions were optimized using the single-factor experiment method and response surface method (RSM). The results showed that the maximum extraction yield of NLCEP was obtained under the following conditions: NaCl solution concentration of 2.96 %, raw material to liquid ratio of 1: 40 g/mL, extraction time of 2 h and extraction temperature of 69 °C. A new novel pure polysaccharide fraction named as NLCEPs-1 was fractionated from NLCEP by using DEAE-Cellulose 52 and Sephadex G-100. Its structure and immunomodulatory and antioxidant activities were analyzed. The results exhibited that the molecular weight of NLCEPs-1 was 31.4 kDa and it was composed of rhamnose, glucose, galactose, xylose and arabinose in the molar percentage of 11.128: 63.770: 5.439: 6.585: 13.077. The backbone of NLCEPs-1 was mainly consisted of → )4-α-d-Glcp (1→, →6)-α-d-Glcp (1→, →1)-β-d-Galp and β-d-Galp-(1→. NLCEPs-1 exhibited the strong antioxidant activity in scavenging ability of various free radicals and immunomodulatory activity by the enhancement of the pinocytic capacity, nitric oxide (NO) and cytokines.

将粗多糖（NLCEP）从提取Notarchus leachii freeri通过将盐蛋字符串 提取 方法。采用单因素 实验优化提取条件 方法和响应面法 (RSM)。结果表明，NLCEP的最大提取率在以下条件下获得：NaCl溶液浓度为2.96%，生液比为1:40 g/mL，提取时间为2 h，提取温度为69 ℃。使用 DEAE-Cellulose 52 和 Sephadex G-100 从 NLCEP 中分离出一种名为 NLCEPs-1 的新型纯多糖级分。分析了其结构和免疫调节和抗氧化活性。结果表明，NLCEPs-1的分子量为31.4 kDa，由鼠李糖、葡萄糖、半乳糖、木糖和阿拉伯糖组成，摩尔百分比为11.128：63.770：5.439：6.585：13.077。NLCEPs-1的主要组成的→骨干）4-α- d -Glcp（1→，→6）-α- d-Glcp (1→, →1)-β- d -Galp 和 β- d -Galp-(1→. NLCEPs-1 通过增强胞饮细胞的功能，在清除各种自由基能力和免疫调节活性方面表现出很强的抗氧化活性。容量、一氧化氮 (NO) 和细胞因子。