A Dopamine Acrylamide Molecule for Promoting Collagen Biomimetic Mineralization and Regulating Crystal Growth Direction,ACS Applied Materials & Interfaces

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A Dopamine Acrylamide Molecule for Promoting Collagen Biomimetic Mineralization and Regulating Crystal Growth Direction
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2021-08-16 , DOI: 10.1021/acsami.1c12412
Leping Wu _{1,

2} , Qingqing Wang ₂ , Yuzhu Li ₂ , Mengmeng Yang ₂ , Menglu Dong ₂ , Xiaoxue He ₂ , Shunli Zheng ₂ , Chris Ying Cao ₂ , Zheng Zhou ₃ , Yuancong Zhao ₄ , Quan-Li Li _{1,

2}

Affiliation

The reconstruction of the intra/interfibrillar mineralized collagen microstructure is extremely important in biomaterial science and regeneration medicine. However, certain problems, such as low efficiency and long period of mineralization, are apparent, and the mechanism of interfibrillar mineralization is often neglected in the present literature. Thus, we propose a novel model of biomimetic collagen mineralization that uses molecules with the dual function of cross-linking collagen and regulating collagen mineralization to construct the intrafibrillar and interfibrillar collagen mineralization of the structure of mineralized collagen hard tissues. In the present study completed in vitro, N-2-(3,4-dihydroxyphenyl) acrylamide (DAA) is used to bind and cross-link collagen molecules and further stabilize the self-assembled collagen fibers. The DAA–collagen complex provides more affinity with calcium and phosphate ions, which can reduce the calcium phosphate/collagen interfacial energy to promote hydroxyapatite (HA) nucleation and accelerate the rate of collagen fiber mineralization. Besides inducing intrafibrillar mineralization, the DAA–collagen complex mineralization template can realize interfibrillar mineralization with the c-axis of the HA crystal on the surface of collagen fibers and between fibers that are parallel to the long axis of collagen fibers. The DAA–collagen complex, as a new type of mineralization template, may provide a new collagen mineralization strategy to produce a mineralized scaffold material for tissue engineering or develop bone-like materials.

中文翻译：

促进胶原仿生矿化并调节晶体生长方向的多巴胺丙烯酰胺分子

纤维内/纤维间矿化胶原微观结构的重建在生物材料科学和再生医学中极其重要。然而，其矿化效率低、矿化周期长等问题也比较突出，而目前的文献往往忽视了纤维间矿化的机理。因此，我们提出了一种新的仿生胶原蛋白矿化模型，利用具有交联胶原蛋白和调节胶原蛋白矿化双重功能的分子来构建矿化胶原蛋白硬组织结构的纤维内和纤维间胶原蛋白矿化。在本体外完成的研究中，N -2-(3,4-二羟基苯基)丙烯酰胺(DAA)用于结合和交联胶原蛋白分子，并进一步稳定自组装胶原纤维。DAA-胶原蛋白复合物与钙离子和磷酸盐离子具有更强的亲和力，可以降低磷酸钙/胶原蛋白界面能，促进羟基磷灰石（HA）成核，加速胶原纤维矿化速率。除了诱导原纤维内矿化外，DAA-胶原蛋白复合物矿化模板还可以实现胶原纤维表面HA晶体的c轴以及平行于胶原纤维长轴的纤维之间的原纤维间矿化。DAA-胶原蛋白复合物作为一种新型矿化模板，可能提供一种新的胶原矿化策略，为组织工程生产矿化支架材料或开发类骨材料。

更新日期：2021-08-25

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