用嵌入抗菌肽的稳定水凝胶涂层包扎血液接触材料，以获得强大的抗菌和抗血栓特性,ACS Applied Materials & Interfaces

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用嵌入抗菌肽的稳定水凝胶涂层包扎血液接触材料，以获得强大的抗菌和抗血栓特性
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2021-08-16 , DOI: 10.1021/acsami.1c05167
Kunpeng Liu ₁ , Fanjun Zhang ₁ , Yuan Wei ₁ , Qinsheng Hu ₂ , Qingfeng Luo ₃ , Chong Chen ₄ , Jingyu Wang ₅ , Li Yang ₁ , Rifang Luo ₁ , Yunbing Wang ₁

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尽管几十年来已经探索了具有强大和协同抗菌和抗血栓特性的血液接触装置敷料，但它仍然是一个巨大的挑战。为了赋予材料显着的抗菌和抗血栓能力，通过磺基甜菜碱甲基丙烯酸酯和丙烯酸在聚合物基材上的表面引发聚合，然后嵌入抗菌肽 (AMP)，包括 WR（序列： WRWRWR-NH ₂ ) 或 Bac2A（序列：RLARIVVIRVAR-NH ₂) AMP。通过 XPS、zeta 电位和 SEM-EDS 测量确定了嵌入 AMP 的水凝胶涂层的化学成分。嵌入 AMP 的防污水凝胶涂层不仅显示出良好的血液相容性，而且对革兰氏阳性菌和革兰氏阴性菌具有优异的杀菌和抗粘连特性。此外，水凝胶涂层可以保护具有长期生物活性的 AMPs 并覆盖点状分布 AMPs 的正电荷，从而很好地保留了散装水凝胶的血液相容性和防污能力。此外，动物实验的微生物学结果也验证了其在体内的抗感染性能。组织学和免疫学数据进一步表明，水凝胶涂层具有优异的抗炎功能。所以，

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Dressing Blood-Contacting Materials by a Stable Hydrogel Coating with Embedded Antimicrobial Peptides for Robust Antibacterial and Antithrombus Properties

Although dressing blood-contacting devices with robust and synergistic antibacterial and antithrombus properties has been explored for several decades, it still remains a great challenge. In order to endow materials with remarkable antibacterial and antithrombus abilities, a stable and antifouling hydrogel coating was developed via surface-initiated polymerization of sulfobetaine methacrylate and acrylic acid on a polymeric substrate followed by embedding of antimicrobial peptides (AMPs), including WR (sequence: WRWRWR-NH₂) or Bac2A (sequence: RLARIVVIRVAR-NH₂) AMPs. The chemical composition of the AMP-embedded hydrogel coating was determined through XPS, zeta potential, and SEM–EDS measurements. The AMP-embedded antifouling hydrogel coating showed not only good hemocompatibility but also excellent bactericidal and antiadhesion properties against Gram-positive and Gram-negative bacteria. Moreover, the hydrogel coating could protect the AMPs with long-term bioactivity and cover the positive charge of the dotted distributed AMPs, which in turn well retained the hemocompatibility and antifouling capacity of the bulk hydrogels. Furthermore, the microbiological results of animal experiments also verified the anti-infection performance in vivo. Histological and immunological data further indicated that the hydrogel coating had an excellent anti-inflammatory function. Therefore, the present study might provide a promising approach to prevent bacterial infections and thrombosis in clinical applications of blood-contacting devices and related implants.

更新日期：2021-08-25

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