荧光成像和元素分析的比较以确定具有强光吸收的无机纳米粒子的生物分布,ACS Applied Materials & Interfaces

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荧光成像和元素分析的比较以确定具有强光吸收的无机纳米粒子的生物分布
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2021-08-16 , DOI: 10.1021/acsami.1c11875
Konstantin Tamarov ₁ , Julie Tzu-Wen Wang ₂ , Juuso Kari ₁ , Emilia Happonen ₁ , Ilkka Vesavaara ₃ , Matti Niemelä ₃ , Paavo Perämäki ₃ , Khuloud T Al-Jamal ₂ , Wujun Xu ₁ , Vesa-Pekka Lehto ₁

Affiliation

黑色多孔硅纳米粒子 (BPSi NPs) 被称为高效红外光吸收剂，非常适用于光热疗法 (PTT) 和光声成像 (PAI)。PTT 和 PAI 需要足够数量的有效吸光 NP 在静脉内给药后在肿瘤中积累。在此，研究了在小鼠静脉内给药后具有不同尺寸（即直径为 140、200 和 300 nm）的聚乙二醇化 BPSi NPs 的生物分布。BPSi NPs 与荧光染料 Cy5.5 和 Cy7.5 结合，分别在体外和体内追踪它们。发现使用体内成像系统 (IVIS)的光学成像不足以评估染料标记的 BPSi NPs 的生物分布在体内，因为 BPSi NPs 固有的强吸光度干扰了荧光检测。这一挑战通过使用电感耦合等离子体发射光谱法来分析离体不同组织和肿瘤中的硅含量。结果表明，大多数聚乙二醇包被的 BPSi NPs 在静脉注射后被发现在肝脏和脾脏中积累。最小的 140 nm 颗粒在肿瘤中积累最多，为注射剂量的 9.5 ± 3.4%（浓度为 0.18 ± 0.08 mg/mL），已知该量可为癌症 PTT 产生足够的热量。此外，本研究的结果还表明，应考虑使用光学成像以外的技术来研究具有强光吸收特性的纳米粒子的器官生物分布。

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Comparison between Fluorescence Imaging and Elemental Analysis to Determine Biodistribution of Inorganic Nanoparticles with Strong Light Absorption

Black porous silicon nanoparticles (BPSi NPs) are known as highly efficient infrared light absorbers that are well-suitable for photothermal therapy (PTT) and photoacoustic imaging (PAI). PTT and PAI require a sufficient number of effectively light-absorbing NPs to be accumulated in tumor after intravenous administration. Herein, biodistribution of PEGylated BPSi NPs with different sizes (i.e., 140, 200, and 300 nm in diameter) is investigated after intravenous administration in mice. BPSi NPs were conjugated with fluorescent dyes Cy5.5 and Cy7.5 to track them in vitro and in vivo, respectively. Optical imaging with an in vivo imaging system (IVIS) was found to be an inadequate technique to assess the biodistribution of the dye-labeled BPSi NPs in vivo because the intrinsic strong absorbance of the BPSi NPs interfered fluorescence detection. This challenge was resolved via the use of inductively coupled plasma optical emission spectrometry to analyze ex vivo the silicon content in different tissues and tumors. The results indicated that most of the polyethylene glycol-coated BPSi NPs were found to accumulate in the liver and spleen after intravenous injection. The smallest 140 nm particles accumulated the most in tumors at an amount of 9.5 ± 3.4% of the injected dose (concentration of 0.18 ± 0.08 mg/mL), the amount known to produce sufficient heat for cancer PTT. Furthermore, the findings from the present study also suggest that techniques other than optical imaging should be considered to study the organ biodistribution of NPs with strong light absorbance properties.

更新日期：2021-09-01

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