Nanozyme has been regarded as an efficient antibiotic to kill bacteria using the reactive oxygen species (ROS) generated by Fenton-like reaction. However, its activity is still unsatisfied and requires large amount of hydrogen peroxide with side effects toward normal tissues. Herein, spherical mesoporous Fe-N-C single-atom nanozyme (SAzyme) is designed for antibacterial therapy via photothermal treatment enhanced Fenton-like catalysis process. Due to the large pore size (4.0 nm), high specific surface area (413.9 m² g⁻¹) and uniform diameter (100 nm), the catalytic performance of Fe-N-C SAzyme is greatly improved. The Michaelis-Menten constant (K_m) is 4.84 mmol L⁻¹, which is similar with that of horseradish peroxidase (3.7 mmol L⁻¹). Moreover, mesoporous Fe-N-C SAzyme shows high photothermal conversion efficiency (23.3 %) owing to the carbon framework. The catalytic activity can be enhanced under light irradiation due to the elevated reaction temperature. The bacteria can also be killed via physical heat effect. Due to the synergistic effect of nanozyme catalysis and photothermal treatment, the antibacterial performance is much higher than that using single antibacterial method. This work provides an alternative for combined antibacterial treatment via photothermal treatment assisted catalytic process using spherical mesoporous single-atom nanozyme as an antibiotic.

Nanozyme 被认为是一种有效的抗生素，可以利用类芬顿反应产生的活性氧 (ROS) 杀死细菌。然而，其活性仍不令人满意，需要大量过氧化氢，对正常组织有副作用。在此，球形介孔 Fe-NC 单原子纳米酶（SAzyme）设计用于通过光热处理增强类芬顿催化过程进行抗菌治疗。由于大孔径（4.0 nm）、高比表面积（413.9 m ² g ^-1）和均匀的直径（100 nm），Fe-NC SAzyme的催化性能大大提高。Michaelis-Menten 常数 ( K _m ) 为 4.84 mmol L ^-1，这与辣根过氧化物酶（3.7 mmol L ^-1）相似。此外，由于碳骨架，介孔 Fe-NC SAzyme 显示出高光热转换效率（23.3%）。由于反应温度升高，可以在光照射下提高催化活性。也可以通过物理热效应杀死细菌。由于纳米酶催化和光热处理的协同作用，抗菌性能远高于使用单一抗菌方法。这项工作为通过使用球形介孔单原子纳米酶作为抗生素的光热处理辅助催化过程进行联合抗菌处理提供了一种替代方法。