Fluorescence Probe for Imaging N-Methyl-d-aspartate Receptors and Monitoring GSH Selectively Using Two-Photon Microscopy,Analytical Chemistry

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Fluorescence Probe for Imaging N-Methyl-d-aspartate Receptors and Monitoring GSH Selectively Using Two-Photon Microscopy
Analytical Chemistry ( IF 6.7 ) Pub Date : 2021-08-12 , DOI: 10.1021/acs.analchem.1c02350
Nahyun Kwon ₁ , Chang Su Lim ₂ , Gyeongju Ko ₁ , Jeongsun Ha ₁ , Dayoung Lee ₁ , Jun Yin ₃ , Hwan Myung Kim ₂ , Juyoung Yoon ₁

Affiliation

N-Methyl-d-aspartate (NMDA) is an excitotoxic amino acid used to identify a specific subset of glutamate receptors. The activity of NMDA receptors is closely related to the redox level of the biological system. Glutathione (GSH) as an antioxidant plays a key role with regard to modulation of the redox environment. In this work we designed and developed a GSH-specific fluorescent probe with the capability of targeting NMDA receptors, which was composed of a two-photon naphthalimide fluorophore, a GSH-reactive group sulfonamide, and an ifenprodil targeting group for the NMDA receptor. This probe exhibited high selectivity toward GSH in comparison to other similar amino acids. Two-photon fluorescence microscopy allowed this probe to successfully monitor GSH in neuronal cells and hippocampal tissues with an excitation at 750 nm. It could serve as a potential practical imaging tool to explore the function of GSH and related biological processes in the brain.

中文翻译：

使用双光子显微镜对 N-甲基-d-天冬氨酸受体进行成像和选择性监测 GSH 的荧光探针

N-甲基-d-天冬氨酸 (NMDA) 是一种兴奋毒性氨基酸，用于识别特定的谷氨酸受体亚群。NMDA受体的活性与生物系统的氧化还原水平密切相关。谷胱甘肽 (GSH) 作为抗氧化剂在调节氧化还原环境方面起着关键作用。在这项工作中，我们设计并开发了一种具有靶向 NMDA 受体能力的 GSH 特异性荧光探针，该探针由双光子萘酰亚胺荧光团、GSH 反应基团磺酰胺和针对 NMDA 受体的艾芬地尔靶向基团组成。与其他类似氨基酸相比，该探针对 GSH 表现出高选择性。双光子荧光显微镜使该探针能够在 750 nm 激发下成功监测神经元细胞和海马组织中的 GSH。

更新日期：2021-08-24

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