Understanding the complex relationship between active small molecules is of great significance in various physiological processes. Herein, we present the design and synthesis of a sequential responsive Lysosome-Naphthalene imide-Azido (lyso-NP-N₃) reporter for probing the H₂S and HOBr within organelle (lysosome) in living cells. Probe lyso-NP-N₃ exhibited high selectivity and sensitivity towards H₂S (LOD = 23.5 nM) and HOBr (LOD = 254 nM). Additionally, lyso-NP-N₃ possessed an excellent lysosome targeting ability and was utilized to visualize the exogenous/endogenous H₂S and HOBr in RAW 264.7, Hela and HepG2 cells. Facilitated by this sequentially activated mechanism, the probe was successfully applied to confirm that the reported scavenger of HOBr, N-acetyl-L-cysteine (NAC) mainly relied on its metabolite H₂S to eliminate excess HOBr, thereby playing the role of cell regulation and protection. These results establish the crosstalk between H₂S and HOBr in lysosome and provide a promising tool to study metabolite interactions.

了解活性小分子之间的复杂关系在各种生理过程中具有重要意义。在此，我们提出了一种顺序响应的溶酶体-萘酰亚胺-叠氮( lyso-NP-N ₃ ) 报告基因的设计和合成，用于探测活细胞细胞器（溶酶体）内的 H ₂ S 和 HOBr。探针lyso-NP-N ₃对 H ₂ S (LOD = 23.5 nM) 和 HOBr (LOD = 254 nM)表现出高选择性和灵敏度。此外，lyso-NP-N ₃具有出色的溶酶体靶向能力，可用于外源/内源 H _{2 的}可视化RAW 264.7、Hela 和 HepG2 细胞中的 S 和 HOBr。在这种顺序激活机制的促进下，该探针成功应用于证实报道的 HOBr 清除剂 N-乙酰-L-半胱氨酸 (NAC) 主要依靠其代谢物 H ₂ S 消除过量的 HOBr，从而发挥细胞的作用。监管和保护。这些结果确定了溶酶体中H ₂ S 和 HOBr之间的串扰，并为研究代谢物相互作用提供了一种很有前景的工具。