Branched Fatty Acid Esters of Hydroxy Fatty Acids Are Preferred Substrates of the MODY8 Protein Carboxyl Ester Lipase,Biochemistry

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Branched Fatty Acid Esters of Hydroxy Fatty Acids Are Preferred Substrates of the MODY8 Protein Carboxyl Ester Lipase
Biochemistry ( IF 2.9 ) Pub Date : 2016-08-10 00:00:00 , DOI: 10.1021/acs.biochem.6b00565
Matthew J. Kolar ₁ , Siddhesh S. Kamat ₂ , William H. Parsons ₂ , Edwin A. Homan ₃ , Tim Maher ₃ , Odile D. Peroni ₄ , Ismail Syed ₄ , Karianne Fjeld _{5,

6} , Anders Molven _{7,

8} , Barbara B. Kahn ₄ , Benjamin F. Cravatt ₂ , Alan Saghatelian ₁

Affiliation

A recently discovered class of endogenous mammalian lipids, branched fatty acid esters of hydroxy fatty acids (FAHFAs), possesses anti-diabetic and anti-inflammatory activities. Here, we identified and validated carboxyl ester lipase (CEL), a pancreatic enzyme hydrolyzing cholesteryl esters and other dietary lipids, as a FAHFA hydrolase. Variants of CEL have been linked to maturity-onset diabetes of the young, type 8 (MODY8), and to chronic pancreatitis. We tested the FAHFA hydrolysis activity of the CEL MODY8 variant and found a modest increase in activity as compared with that of the normal enzyme. Together, the data suggest that CEL might break down dietary FAHFAs.

中文翻译：

羟基脂肪酸的支链脂肪酸酯是MODY8蛋白羧基酯脂肪酶的优选底物

最近发现的一类内源性哺乳动物脂质，羟基脂肪酸的支链脂肪酸酯（FAHFA），具有抗糖尿病和抗炎活性。在这里，我们确定并验证了羧基酯脂肪酶（CEL）（一种水解胆固醇酯和其他饮食脂质的胰酶）作为FAHFA水解酶。CEL的变体与年轻的8型青年发病型糖尿病（MODY8）和慢性胰腺炎有关。我们测试了CEL MODY8变体的FAHFA水解活性，发现与正常酶相比，活性有所增加。总体而言，数据表明CEL可能会分解饮食中的FAHFAs。

更新日期：2016-08-10

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