Single-Cell Imaging of m6A-Modified RNA Using m6A-Specific In Situ Hybridization-Mediated Proximity Ligation Assay (m6AISH-PLA),Angewandte Chemie International Edition

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Single-Cell Imaging of m6A-Modified RNA Using m6A-Specific In Situ Hybridization-Mediated Proximity Ligation Assay (m6AISH-PLA)
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2021-08-09 , DOI: 10.1002/anie.202110138
Xiaojun Ren _{1,

2} , Ruijie Deng ₁ , Kaixiang Zhang ₁ , Yupeng Sun ₁ , Yue Li ₁ , Jinghong Li ₁

Affiliation

N6-methyladenosine (m⁶A)-modified RNA analysis is limited at the bulk-population level, thus not giving access to spatiotemporal and cell-to-cell variability information. In their Communication (DOI: 10.1002/anie.202109118), Jinghong Li et al. propose a powerful in situ imaging method termed m⁶A-specific in situ hybridization mediated proximity ligation assay (m⁶AISH-PLA), which enables the identification of m⁶A modifications at a specific location in RNAs and the imaging of m⁶A RNA with single-cell and single-molecule resolution.

中文翻译：

使用 m6A 特异性原位杂交介导的邻近连接分析 (m6AISH-PLA) 对 m6A 修饰的 RNA 进行单细胞成像

N6-甲基腺苷 (m ⁶ A) 修饰的 RNA 分析仅限于群体水平，因此无法获得时空和细胞间变异性信息。在他们的通讯（DOI：10.1002/anie.202109118）中，Jinghong Li 等人。提出了一种强大的原位成像方法，称为 m ⁶ A 特异性原位杂交介导的邻近连接测定 (m ⁶ AISH-PLA)，它能够识别RNA 中特定位置的 m ⁶ A 修饰和 m ⁶ A的成像具有单细胞和单分子分辨率的 RNA。

更新日期：2021-08-09

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