The neuroprotective activity of 2-heptyl-3-hydroxy-4(1H)-quinolone (compound 1) was evaluated using the neurotoxicity of glutamate in the HT22 cell line. Compound 1, known as a signal molecule of the bacterial quorum-sensing system, protects neuronal cells from glutamate-induced neurotoxicity by inhibiting cellular Ca²⁺ uptake and glutamate-triggered ROS accumulation. MAPK signaling pathway inhibition by compound 1 was evaluated by immunoblotting the phosphorylation status of the proteins. Furthermore, pro-apoptotic protein levels and AIF translocation to the nucleus were found to be reduced by compound 1. In conclusion, compound 1 showed neuroprotective effects by inhibiting apoptotic neuronal cell death.

使用 HT22 细胞系中谷氨酸的神经毒性评估 2-庚基-3-羟基-4(1H)-喹诺酮（化合物 1）的神经保护活性。化合物 1 被称为细菌群体感应系统的信号分子，通过抑制细胞 Ca ²⁺摄取和谷氨酸触发的 ROS 积累，保护神经元细胞免受谷氨酸诱导的神经毒性。通过免疫印迹蛋白质的磷酸化状态来评估化合物 1 对 MAPK 信号通路的抑制作用。此外，发现化合物1可降低促凋亡蛋白水平和AIF向细胞核的易位。总之，化合物1通过抑制凋亡性神经元细胞死亡而显示出神经保护作用。