Gastric cancer (GC) is one of the most common malignancies and has the second highest lethal rate in the world; thus, finding new medicines with high potency and low toxicity is urgent. (Carr.) Bur. ex Lavallee (Moraceae) is a traditional medicinal herb that is considered to have antitumour efficacy. We extracted and isolated cudraxanthone L (CXL) from and evaluated its anti-cancer efficacy. CXL treatment inhibited angiogenesis of chorioallantoic membrane (CAM) and repressed the cell viability of various human cancer cells, indicating it presented the antitumour potential. Among them, CXL presented the best inhibitory effects on MGC803 cells. In addition, the invasion, migration and clonogenicity were significantly repressed, S phase of the cell cycle was arrested, and apoptosis was induced when MGC803 cells were treated with CXL. The results of RNA sequencing, qRT-PCR and western blotting verified that CXL regulated the MAPK signalling pathway and induced apoptosis by FAS-mediated pathway. The data revealed that CXL arrested tumour growth without toxic effects and upregulated the protein levels in FAS-mediated pathway in MGC803 gastric cancer-bearing mice. In summary, we demonstrate CXL presents impactful anti-GC efficacy by regulating the MAPK signalling pathway and promoting the FAS-mediated pathway.

中文翻译：

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Cudraxanthone L 通过调节 MAPK 信号和促进 FAS 介导的通路抑制胃癌


胃癌（GC）是最常见的恶性肿瘤之一，死亡率位居世界第二；因此，寻找高效低毒的新药刻不容缓。 （卡尔）布尔。 ex Lavallee（桑科）是一种传统药草，被认为具有抗肿瘤功效。我们从其中提取并分离了 cudraxanthone L (CXL) 并评估了其抗癌功效。 CXL 治疗可抑制绒毛尿囊膜 (CAM) 的血管生成，并抑制多种人类癌细胞的细胞活力，表明其具有抗肿瘤潜力。其中CXL对MGC803细胞的抑制效果最好。此外，当用CXL处理MGC803细胞时，侵袭、迁移和克隆形成被显着抑制，细胞周期的S期被阻滞，并诱导细胞凋亡。 RNA测序、qRT-PCR和蛋白质印迹结果证实CXL调节MAPK信号通路并通过FAS介导的通路诱导细胞凋亡。数据显示，CXL 在没有毒性作用的情况下抑制肿瘤生长，并上调 MGC803 胃癌小鼠中 FAS 介导的通路中的蛋白质水平。总之，我们证明 CXL 通过调节 MAPK 信号通路和促进 FAS 介导的通路而具有有效的抗 GC 功效。