柠檬酸功能化的铑-铂纳米颗粒作为过氧化物酶模拟物用于测定胆固醇,ACS Applied Nano Materials

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柠檬酸功能化的铑-铂纳米颗粒作为过氧化物酶模拟物用于测定胆固醇
ACS Applied Nano Materials ( IF 5.3 ) Pub Date : 2021-07-29 , DOI: 10.1021/acsanm.1c01457
Seong Eun Son ₁ , Pramod K. Gupta ₁ , Won Hur ₁ , Han Been Lee ₁ , Yosep Park ₁ , Jiyeon Park ₁ , Seong Nyeon Kim ₁ , Gi Hun Seong ₁

Affiliation

纳米结构的人工酶，也称为纳米酶，被认为是天然酶的有效替代品。在本研究中，通过简单的一锅法合成了柠檬酸功能化的铑-铂纳米粒子（CA-RhPt NPs）。透射电子显微镜图像显示 CA-RhPt NPs 的均匀结构，平均直径为 3.5 ± 0.5 nm，X 射线光电子能谱和 zeta 电位测量表明 CA-RhPt NPs 中存在柠檬酸。由于 Rh 和 Pt 的协同作用，CA-RhPt NPs 表现出增强的过氧化物酶样活性，催化 3,3',5,5'-四甲基联苯胺 (TMB) 和过氧化氢 (H ₂ O ₂ ) 之间的反应。发现CA-RhPt NPs分解H的催化机制₂ O ₂生成羟基自由基 (•OH)，然后•OH 氧化 TMB，生成蓝色产物。然后将优异的过氧化物酶样 CA-RhPt NPs 应用于胆固醇的比色测定。基于 CA-RhPt NP 的比色法在 0.08 至 5.0 mM 范围内显示出灵敏的胆固醇检测，检测限为 25.7 μM。加标人血清样品中胆固醇浓度的回收率分别为 96.8% 和 106%，与商业化胆固醇检测试剂盒的回收率相当。这些结果证明了 CA-RhPt NPs 作为天然酶替代品的可能性和敏感胆固醇检测的适用性。

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Citric Acid-Functionalized Rhodium–Platinum Nanoparticles as Peroxidase Mimics for Determination of Cholesterol

Nanostructured artificial enzymes, also called nanozymes, are considered effective substitutes for natural enzymes. In this study, citric acid-functionalized rhodium–platinum nanoparticles (CA-RhPt NPs) were synthesized by a simple one-pot synthetic method. Transmission electron microscopy images showed the uniform structure of the CA-RhPt NPs, with an average diameter of 3.5 ± 0.5 nm, and X-ray photoelectron spectroscopy and zeta potential measurements demonstrated the presence of citric acid in the CA-RhPt NPs. The CA-RhPt NPs exhibited enhanced peroxidase-like activity that catalyzed the reaction between 3,3′,5,5′-tetramethylbenzidine (TMB) and hydrogen peroxide (H₂O₂) due to the synergistic effects of Rh and Pt. The catalytic mechanism of CA-RhPt NPs was found to decompose H₂O₂ to a hydroxyl radical (•OH), and then, the •OH oxidizes the TMB, generating a blue product. The excellent peroxidase-like CA-RhPt NPs were then applied to the colorimetric determination of cholesterol. The CA-RhPt NP-based colorimetry showed sensitive cholesterol detection in the range from 0.08 to 5.0 mM, with a detection limit of 25.7 μM. The recoveries of cholesterol concentrations from the spiked human serum samples were 96.8% and 106%, which were comparable to those from the commercialized cholesterol detection kit. These results demonstrate the possibility of CA-RhPt NPs as alternatives for natural enzymes and applicability for sensitive cholesterol detection.

更新日期：2021-08-27

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