Jute sticks obtained after the extraction of jute fiber are an excellent biomass feedstock with a significant amount of carbohydrates that makes it an attractive resource for sustainable energy generation. However, the high lignin content in the jute stick hinders the cellulosic component of the cell wall from enzymatic hydrolysis.This work demonstrates the lignin degradation of jute stick biomass by Trametes maxima laccase in the presence of mediator Hydroxybenzotriazole and improvement in its subsequent saccharification. Lignin component in jute stick is reduced by 21.8% in a single reaction treatment with laccase-mediator compared to the untreated jute stick sample used as control. The yield of fermentable sugar is increased by 19.5% that verifies enhanced saccharification after lignin removal. Delignification of jute stick was corroborated through different analytical techniques. The Pyrolysis gas chromatography/mass spectrometry results further confirms abundance of S lignin unit in the jute stick compared to the H and G unit and modification in lignin polymer as a change in the syringyl-to-guaiacyl ratio. Hence, this work demonstrates that jute stick can be effectively delignified using biocatalyst-mediator system and utilized as biomass source, thus contributing in circular bio-economy through waste valorization.

黄麻纤维提取后获得的黄麻棒是一种极好的生物质原料，含有大量碳水化合物，使其成为可持续能源生产的有吸引力的资源。然而，黄麻棒中的高木质素含量阻碍了细胞壁纤维素成分的酶水解。 这项工作证明了最大栓菌对黄麻棒生物质的木质素降解漆酶在介体羟基苯并三唑的存在下并改善其随后的糖化。与用作对照的未经处理的黄麻棒样品相比，在使用漆酶介体的单一反应处理中，黄麻棒中的木质素成分减少了 21.8%。可发酵糖的产量增加了 19.5%，证明去除木质素后糖化作用增强。通过不同的分析技术证实了黄麻棒的脱木素。热解气相色谱/质谱结果进一步证实，与 H 和 G 单元相比，黄麻棒中 S 木质素单元的丰度以及木质素聚合物的改性是丁香基与愈创木酰基比率的变化。因此，这项工作表明黄麻棒可以使用生物催化剂-介体系统有效地脱木素并用作生物质来源，