用于高度稳健和高效 DNA 吸附和传感的金属掺杂聚多巴胺纳米颗粒,Langmuir

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用于高度稳健和高效 DNA 吸附和传感的金属掺杂聚多巴胺纳米颗粒
Langmuir ( IF 3.7 ) Pub Date : 2021-07-26 , DOI: 10.1021/acs.langmuir.1c00783
Mohamad Zandieh ₁ , Juewen Liu ₁

Affiliation

控制纳米材料上的 DNA 吸附对于分析和生物医学科学的广泛应用至关重要。聚多巴胺 (PDA) 是一种多功能材料，几乎可以涂覆在任何表面上，因此将 DNA 吸附到 PDA 上可以成为表面间接 DNA 功能化的通用方法。据报道，多价金属离子可促进 PDA 纳米粒子 (NPs) 上的 DNA 吸附，但之前的工作是在 PDA 形成后添加金属离子。在此，我们比较了 PDA NPs 合成过程中添加的多价金属离子（称为金属掺杂）与合成后添加的多价金属离子（金属吸附）的影响。Ca ²⁺、Zn ²⁺、Ni ²⁺、Fe ³⁺、Gd等一系列金属离子³⁺进行了测试，并详细研究了Zn ²⁺由于其优异的促进 DNA 吸附的能力。对于 100 μM Zn ²⁺，金属掺杂的 NPs 比金属吸附的 NPs 在缓冲液中吸附 DNA 的效率高约 30%，这归因于金属掺杂的 NPs 表面上更高的金属负载。金属掺杂的 NPs 的金属浸出可以忽略不计，并且它们显示出比金属吸附的 NPs 显着更高的稳健性，导致从血清中提取 DNA 的效率提高了 20 倍。基于解吸研究，证实了对金属掺杂的 NPs 具有更高的吸附亲和力。最后，Zn ²⁺掺杂的 PDA NPs 用于灵敏的 DNA 检测，检测限为 0.45 nM，传感器对非特异性蛋白质和磷酸盐置换具有高度抵抗力。

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Metal-Doped Polydopamine Nanoparticles for Highly Robust and Efficient DNA Adsorption and Sensing

Controlling DNA adsorption on nanomaterials is crucial for a wide range of applications in analytical and biomedical sciences. Polydopamine (PDA) is a versatile material that can be coated on nearly any surface, and thus adsorbing DNA onto PDA can be a general method for indirect DNA functionalization of surfaces. Polyvalent metal ions were reported to promote DNA adsorption on PDA nanoparticles (NPs), but previous works added the metal ions after the formation of PDA. Herein, we compared the effect of polyvalent metal ions added during the synthesis of PDA NPs (called metal-doped) with the effect of polyvalent metal ions added after the synthesis (metal-adsorbed). A series of metal ions including Ca²⁺, Zn²⁺, Ni²⁺, Fe³⁺, and Gd³⁺ were tested, and Zn²⁺ was studied in detail due to its excellent ability for promoting DNA adsorption. With 100 μM Zn²⁺, metal-doped NPs were ∼30% more efficient than metal-adsorbed NPs for DNA adsorption in buffer attributable to a higher metal loading on the surface of the metal-doped NPs. Metal leaching was negligible from the metal-doped NPs, and they showed a remarkably higher robustness than the metal-adsorbed NPs, resulting in a 20-fold higher DNA extraction efficiency from serum. Based on the desorption studies, a higher adsorption affinity for the metal-doped NPs was confirmed. Finally, the Zn²⁺-doped PDA NPs were used for sensitive DNA detection with a limit of detection of 0.45 nM, and the sensor was highly resistant to nonspecific protein and phosphate displacement.

更新日期：2021-08-03

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