Digestion of pectic polysaccharide from Brassica rapa L. in vitro and its effect on the intestinal microbiota in cyclophosphamide-treated mice,International Journal of Food Science & Technology

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Digestion of pectic polysaccharide from Brassica rapa L. in vitro and its effect on the intestinal microbiota in cyclophosphamide-treated mice
International Journal of Food Science & Technology ( IF 2.6 ) Pub Date : 2021-07-22 , DOI: 10.1111/ijfs.15278
Wei Wang ₁ , Lijun Fan ₁ , Xuewen Li ₁ , Zirong Wang ₁ , Jianzhong Zhou ₁ , Buligen Jialengbieke ₁ , Jianye Ren ₁ , Xiaodong Hu ₁

Affiliation

In this study, the digestion of pectic polysaccharide from Brassica rapa L. (BRP) in vitro and its effect on the intestinal microbiota in cyclophosphamide (Cy)-treated mice were investigated. As a result, the molecular weight (M_w) of BRP was significantly decreased after simulated gastrointestinal digestion, and content of reducing sugars (C_R) was significantly increased (P < 0.05), implying that BRP was gradually degraded under gastrointestinal digestion conditions in vitro. In vivo, both 50 or 200 mg kg⁻¹·BW/d of BRP significantly improved SCFAs concentrations that decreased by Cy treatment (P < 0.05). Meanwhile, BRP enriched the alpha-diversity of intestinal microbiota and altered the microbial composition with an increase of Lactobacillus and a decrease of Clostridium, Ruminococcus, Oscillospira (P < 0.05). Besides, BRP intervention was conducive to the recovery of mouse body weight, thymus and spleen indices, and contributed to improve the intestinal barrier.

中文翻译：

芸苔中果胶多糖的体外消化及其对环磷酰胺处理小鼠肠道微生物群的影响

在这项研究中，研究了芸苔中果胶多糖(BRP)的体外消化及其对环磷酰胺 (Cy) 处理小鼠肠道微生物群的影响。其结果是，分子量（中号_瓦特）BRP物显著模拟胃肠消化后下降，和还原糖（内容Ç _{- [R ）}中的溶液显著增加（P <0.05），这意味着BRP逐渐胃肠消化条件下降解在体外。在体内，50 或 200 mg kg ^-1 ·BW/d 的 BRP 均显着提高了 SCFAs 浓度，而后者因 Cy 处理而降低（P < 0.05)。同时，BRP丰富了肠道微生物群的α-多样性，改变了微生物组成，增加了乳酸杆菌，减少了梭状芽孢杆菌、瘤胃球菌、Oscillospira（P < 0.05）。此外，BRP干预有利于小鼠体重、胸腺和脾脏指标的恢复，有助于改善肠道屏障。

更新日期：2021-07-22

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