The Polymeric Matrix Composition of Vibrio cholerae Biofilms Modulate Resistance to Silver Nanoparticles Prepared by Hydrothermal Synthesis,ACS Applied Materials & Interfaces

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The Polymeric Matrix Composition of Vibrio cholerae Biofilms Modulate Resistance to Silver Nanoparticles Prepared by Hydrothermal Synthesis
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2021-07-21 , DOI: 10.1021/acsami.1c07455
Clémence Abriat _{1,

2} , Olivier Gazil ₁ , Marie-Claude Heuzey ₁ , France Daigle ₂ , Nick Virgilio ₁

Affiliation

Biofilms represent the dominant microbial lifestyle in nature. These complex microbial communities in which bacteria are embedded in a self-produced protective polymeric extracellular matrix, display an enhanced resistance to antimicrobials and thus represent a major health challenge. Although nanoparticles have proven to be effective against bacteria, the interactions between nanoparticles and the polymeric biofilm matrix are still unclear. In this work, silver nanoparticles (AgNPs) were used on mature biofilms formed by the pathogen Vibrio cholerae, and their effects on the biofilm microstructure were evaluated. Bacteria cells within mature biofilms showed an increased tolerance to AgNPs, with their elimination requiring a concentration nine times higher than planktonic cells. Mutant strains not able to form a pellicle biofilm were four times more susceptible to AgNPs than the wild-type strain forming a strong biofilm. Moreover, electron microscopy analysis revealed that AgNPs interacted with the extracellular matrix components and disrupted its microstructure. Finally, two major proteins, Bap1 and RbmA, appeared to mediate the biofilm bacterial resistance to AgNPs. This work highlights the role of the polymeric biofilm matrix composition in resistance to AgNPs. It underlines how crucial it is to understand and characterize the interactions between nanoparticles and the biofilm matrix, in order to design appropriate metallic nanoparticles efficient against bacterial biofilms.

中文翻译：

霍乱弧菌生物膜的聚合物基质组合物调节对水热合成制备的银纳米颗粒的抵抗力

生物膜代表了自然界中占主导地位的微生物生活方式。这些复杂的微生物群落中细菌嵌入自产的保护性聚合物细胞外基质中，对抗菌剂的抗性增强，因此对健康构成了重大挑战。尽管纳米颗粒已被证明对细菌有效，但纳米颗粒与聚合物生物膜基质之间的相互作用仍不清楚。在这项工作中，银纳米粒子 (AgNPs) 被用于由病原体霍乱弧菌形成的成熟生物膜，并评估了它们对生物膜微结构的影响。成熟生物膜内的细菌细胞对 AgNPs 的耐受性增加，消除它们需要比浮游细胞高 9 倍的浓度。无法形成薄膜生物膜的突变菌株对 AgNPs 的敏感性是形成强生物膜的野生型菌株的四倍。此外，电子显微镜分析表明，AgNPs 与细胞外基质成分相互作用并破坏了其微观结构。最后，两种主要蛋白质 Bap1 和 RbmA 似乎介导了生物膜细菌对 AgNPs 的抗性。这项工作突出了聚合物生物膜基质组合物在抗 AgNPs 中的作用。它强调了理解和表征纳米粒子与生物膜基质之间的相互作用是多么重要，

更新日期：2021-08-04

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