Effects of trypsin-induced limited hydrolysis on the structural, functional, and bioactive properties of sericin,RSC Advances

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Effects of trypsin-induced limited hydrolysis on the structural, functional, and bioactive properties of sericin
RSC Advances ( IF 3.9 ) Pub Date : 2021-07-21 , DOI: 10.1039/d1ra03772b
Adil Omar _{1,

2} , Yanhua Gao ₁ , Atikan Wubulikasimu _{1,

2} , Amina Arken _{1,

2} , Haji Akber Aisa ₁ , Abulimiti Yili ₁

Affiliation

The effects of trypsin-induced hydrolysis on the structural, functional, and antioxidant properties of sericin were studied. The structural properties of sericin and its hydrolysates were characterized by using SDS-PAGE, SEC-HPLC, surface hydrophobicity, and circular dichroism. Antioxidative properties were evaluated based on quenching capacity against hydroxyl, DPPH, and ABTS, and metal (Fe²⁺, Cu²⁺) chelating activity. The enzymatic hydrolysis raised the flexibility, changed emulsifying and foaming properties, and improved the solubility and antioxidant activity of sericin. Meanwhile, the hydrolysis led to a decline in gelation capacity, oil holding capacity, and water holding capacity. Sericin and its hydrolysates exhibited excellent function with regard to oil holding, emulsifying, and foaming. Sericin and its hydrolysates had clear effects on the growth of both Enterococcus faecalis and Lactobacillus bulgaricus strains.

中文翻译：

胰蛋白酶诱导的有限水解对丝胶蛋白结构、功能和生物活性特性的影响

研究了胰蛋白酶诱导的水解对丝胶的结构、功能和抗氧化特性的影响。采用SDS-PAGE、SEC-HPLC、表面疏水性和圆二色性表征丝胶及其水解产物的结构特性。基于对羟基、DPPH和ABTS以及金属（Fe ²⁺、Cu ^{2+ ）的淬灭能力评估抗氧化性能}) 螯合活性。酶解提高了丝胶的柔韧性，改变了乳化和发泡性能，提高了丝胶的溶解度和抗氧化活性。同时，水解导致凝胶能力、持油能力和持水能力下降。丝胶及其水解物在保油、乳化和起泡方面表现出优异的性能。丝胶及其水解物对粪肠球菌和保加利亚乳杆菌菌株的生长都有明显的影响。

更新日期：2021-07-21

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