In this study, tailored-made citrus pectin-derived compounds were produced through controlled enzymatic and/or chemical modifications of commercial citrus pectin with different degrees of methylesterification (DM) and similar average molecular weight (M_W). In the first treatment, degradation of the citrus pectin (CP) materials by endo-polygalacturonase (EPG) yielded pectins with average M_w’s (between 2 and 60 kDa). Separation and identification of the oligosaccharide fraction present in these samples, revealed the presence of non-methylesterified galacturonic acid oligomers with degree of polymerization (DP) 1–5. In the second treatment, exploiting the combined effect of EPG and pectin lyase, compounds with M_W between 2 and 21 kDa, containing methylesterified and non-methylesterified polygalacturonans (DP 1–6), were generated. Finally, CP was sequentially modified by chemical saponification and the action of EPG. A sample of DM 11% and M_W 2.7 kDa, containing POS (DP 1–5), was produced. Diverse pectin-derived compounds were successfully generated for further studies exploring their functionality.

在这项研究中，通过具有不同程度的甲酯化（DM）和类似均分子量（M商业柑桔果胶的控制的酶和/或化学修饰产生定制制造的柑橘果胶衍生的化合物_{w ^}）。在第一处理，由柑橘果胶降解（CP）的材料内-polygalacturonase（EPG）产生了具有平均中号果胶_瓦特的（2和60 kDa的之间）。这些样品中存在的低聚糖部分的分离和鉴定表明存在聚合度 (DP) 1-5 的非甲基酯化半乳糖醛酸低聚物。在第二个处理中，利用 EPG 和果胶裂解酶的联合作用，具有 M _{W 的}化合物产生了 2 到 21 kDa，包含甲基酯化和非甲基酯化的聚半乳糖醛酸 (DP 1-6)。最后，通过化学皂化和EPG的作用依次修饰CP。生产了一个 DM 11% 和 M _W 2.7 kDa的样品，含有 POS (DP 1-5)。成功地生成了多种果胶衍生化合物，用于进一步研究探索其功能。