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Native Bacterial Community Convergence in Augmented and Stimulated Ureolytic MICP Biocementation
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2021-07-19 , DOI: 10.1021/acs.est.1c01520
Charles M R Graddy 1 , Michael G Gomez 2 , Jason T DeJong 3 , Douglas C Nelson 1
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2021-07-19 , DOI: 10.1021/acs.est.1c01520
Charles M R Graddy 1 , Michael G Gomez 2 , Jason T DeJong 3 , Douglas C Nelson 1
Affiliation
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Microbially induced calcite precipitation is a biomineralization process with numerous civil engineering and ground improvement applications. In replicate soil columns, the efficacy and microbial composition of soil bioaugmented with the ureolytic bacterium Sporosarcina pasteurii were compared to a biostimulation method that enriches native ureolytic soil bacteria in situ under conditions analogous to field implementation. The selective enrichment resulting from sequential stimulation treatments strongly selected for Firmicutes (>97%), with Sporosarcina and Lysinibacillus comprising 60 to 94% of high-throughput 16S rDNA sequences in each suspended community sample. Seven species of the former and two of the latter were present in greater than 10% abundance at different times, demonstrating unexpected within-genus diversity and robustness in the suspended phase of this highly selective environment. Based on longer 16S sequences, it was inferred that augmented S. pasteurii competed poorly with natural bacteria, decreasing to below detection after nine treatments, while the native microbial community was enriched to approximately that present in the stimulated columns. These analyses were corroborated by the observed convergence in bulk ureolytic rates and calcite contents between techniques. However, a 10-fold discrepancy between the observed cell density and an activity-based estimate indicates the attached community, uncharacterized despite efforts, substantially contributes to bulk behavior.
中文翻译：
增强和刺激的尿素分解 MICP 生物水泥中的天然细菌群落会聚
微生物诱导方解石沉淀是一种生物矿化过程，具有众多土木工程和地面改善应用。在重复的土壤柱中，将用尿素分解细菌Sporosarcina pasteurii进行生物增强的土壤的功效和微生物组成与在类似于田间实施的条件下原位富集天然尿素分解土壤细菌的生物刺激方法进行了比较。连续刺激处理产生的选择性富集强烈选择用于厚壁菌 (>97%)，孢子藻和赖氨酸杆菌每个悬浮群落样本中包含 60% 到 94% 的高通量 16S rDNA 序列。前者的 7 个物种和后者的 2 个物种在不同时间的丰度超过 10%，表明在这种高度选择性环境的悬浮相中出乎意料的属内多样性和稳健性。基于更长的 16S 序列，推断增加了巴氏酵母菌与天然细菌的竞争很差，在九次处理后降低到低于检测水平，而原生微生物群落被丰富到大约存在于刺激柱中。这些分析得到了观察到的大量尿素分解率和技术之间方解石含量的收敛性的证实。然而，观察到的细胞密度和基于活动的估计值之间的 10 倍差异表明，尽管付出了努力，但仍然没有表征的附着群落对大量行为产生了重大影响。
更新日期：2021-08-03
中文翻译：
增强和刺激的尿素分解 MICP 生物水泥中的天然细菌群落会聚
微生物诱导方解石沉淀是一种生物矿化过程，具有众多土木工程和地面改善应用。在重复的土壤柱中，将用尿素分解细菌Sporosarcina pasteurii进行生物增强的土壤的功效和微生物组成与在类似于田间实施的条件下原位富集天然尿素分解土壤细菌的生物刺激方法进行了比较。连续刺激处理产生的选择性富集强烈选择用于厚壁菌 (>97%)，孢子藻和赖氨酸杆菌每个悬浮群落样本中包含 60% 到 94% 的高通量 16S rDNA 序列。前者的 7 个物种和后者的 2 个物种在不同时间的丰度超过 10%，表明在这种高度选择性环境的悬浮相中出乎意料的属内多样性和稳健性。基于更长的 16S 序列，推断增加了巴氏酵母菌与天然细菌的竞争很差，在九次处理后降低到低于检测水平，而原生微生物群落被丰富到大约存在于刺激柱中。这些分析得到了观察到的大量尿素分解率和技术之间方解石含量的收敛性的证实。然而，观察到的细胞密度和基于活动的估计值之间的 10 倍差异表明，尽管付出了努力，但仍然没有表征的附着群落对大量行为产生了重大影响。
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