使用来自超声成像的结构先验信息的小动物 360 度荧光漫反射光学断层扫描。,Journal of Biomedical Optics

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使用来自超声成像的结构先验信息的小动物 360 度荧光漫反射光学断层扫描。
Journal of Biomedical Optics ( IF 3.0 ) Pub Date : 2020-03-01 , DOI: 10.1117/1.jbo.25.3.036001
Pei-An Lo ₁ , Shih-Po Su ₁ , Huihua Kenny Chiang ₁

Affiliation

我们展示了自由空间荧光漫反射光学断层扫描 (FDOT) 和手持超声 (US) 成像的双重模式，以揭示小动物的功能和结构信息。FDOT 是一种非侵入性方法，用于从表面的光分布检查物体内部的荧光团。在 FDOT 中，660 nm 连续波二极管激光器用作激发源，电子倍增电荷耦合器件 (EMCCD) 用于荧光数据采集。激光器和 EMCCD 都安装在 360 度旋转台架上，用于传输光学数据收集。结构信息是通过单侧访问从 6 到 17 MHz 手持式美国线性换能器获得的，并在重建中作为软先验进行。旋转范围从0度到360度；不同的旋转度数，确定物体位置和参数以进行比较。体模和组织体模结果都表明，使用该成像系统可以准确定量地恢复荧光团分布。最后，一项动物研究证实该系统可以提取双模态图像，验证其在进一步体内实验中的可行性。在所有实验中，误差和标准偏差随着旋转度数的增加而减小，当旋转度数增加超过 135 度时，误差减小到 10%。验证其进一步体内实验的可行性。在所有实验中，误差和标准偏差随着旋转度数的增加而减小，当旋转度数增加超过 135 度时，误差减小到 10%。验证其进一步体内实验的可行性。在所有实验中，误差和标准偏差随着旋转度数的增加而减小，当旋转度数增加超过 135 度时，误差减小到 10%。

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Small-animal 360-deg fluorescence diffuse optical tomography using structural prior information from ultrasound imaging.

We demonstrate dual modality of free-space fluorescence diffuse optical tomography (FDOT) and handheld ultrasound (US) imaging to reveal both functional and structural information in small animals. FDOT is a noninvasive method for examining the fluorophore inside an object from the light distribution of the surface. In FDOT, a 660-nm continuous wave diode laser was used as an excitation source and an electron-multiplying charge-coupled device (EMCCD) was used for fluorescence data acquisition. Both the laser and EMCCD were mounted on a 360-deg rotation gantry for the transmission optical data collection. The structural information is obtained from a 6- to 17-MHz handheld US linear transducer by single-side access and conducts in the reconstruction as soft priors. The rotation ranges from 0 deg to 360 deg; different rotation degrees, object positions, and parameters were determined for comparison. Both phantom and tissue phantom results demonstrate that fluorophore distribution can be recovered accurately and quantitatively using this imaging system. Finally, an animal study confirms that the system can extract a dual-modality image, validating its feasibility for further in vivo experiments. In all experiments, the error and standard deviation decrease as the rotation degree is increased and the error was reduced to 10% when the rotation degree was increased over 135 deg.

更新日期：2020-03-01

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