Abstract

Extensive phytochemical investigation of Schisandra sphenanthera leaves resulted in the isolation of six highly oxygenated nortriterpenoids (1–6) and five lignans (7–11) including a new pre-schisanartane-type, schisandrathera A (1), a new dibenzocyclooctadiene glycoside, schisandrathera B (7) and two new lignans, schisandrathera C (8) and schisandrathera D (9). Their chemical structures including absolute configurations were determined extensively by means of HR-ESI-MS, NMR, and ECD spectra. In addition, all isolated compounds were tested for cytotoxic activity against PC3 (prostate cancer) and MCF7 (breast cancer) cell lines. Among these compounds, schirubrisin B (3) showed strong cytotoxic effect on both PC3 and MCF7 cell lines with IC₅₀ values of 3.21 ± 0.68, 13.30 ± 0.68 μM, respectively, whereas ten remaining compounds were found to be less effective in the investigated models.

摘要

对华中五味子叶进行广泛的植物化学研究，结果分离出六种高度氧化的去甲三萜类化合物 ( 1-6 ) 和五种木脂素 ( 7-11 )，包括一种新的前五味子烷型五味子 A ( 1 )、一种新的二苯并环辛二烯苷、五味子B ( 7 ) 和两种新的木脂素，五味子 C ( 8 ) 和五味子 D ( 9）。它们的化学结构（包括绝对构型）通过 HR-ESI-MS、NMR 和 ECD 光谱广泛确定。此外，测试了所有分离的化合物对 PC3（前列腺癌）和 MCF7（乳腺癌）细胞系的细胞毒活性。在这些化合物中，schirubrisin B ( 3 ) 对 PC3 和 MCF7 细胞系均显示出强烈的细胞毒性作用，IC ₅₀值分别为 3.21 ± 0.68、13.30 ± 0.68 μM，而其余十种化合物在所研究的模型中效果较差.