The effects of 4-amino-3,5,6-trichloropicolinic acid (picloram) on protein synthesis in bean (Phaseolus vulgaris L. cv. ‘Astro’) hypocotyl and hook tissues were studied. Picloram (10-4M) was shown to have a stimulatory effect on 14C-1-DL-leucine uptake in hook but not hypocotyl tissues. Maximum leucine incorporation and maximum total protein concentration occurred in hook tissues treated with 10-4M picloram. Inhibition of protein synthesis with cycloheximide (CH) and erythromycin (ERY) indicates that endogenous and picloram-stimulated protein synthesis is a function of the 80S cytoplasmic ribosomes rather than 70S chloroplast or mitochondria ribosomes.

中文翻译：

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4-氨基-3,5,6-三氯吡啶甲酸对蛋白质合成的影响

研究了 4-氨基-3,5,6-三氯吡啶甲酸 (picloram) 对豆 (Phaseolus vulgaris L. cv. 'Astro') 下胚轴和钩组织中蛋白质合成的影响。Picloram (10-4M) 显示对钩子中的 14C-1-DL-亮氨酸摄取有刺激作用，但对下胚轴组织没有刺激作用。在用 10-4M 毒莠定处理的钩组织中出现最大亮氨酸掺入和最大总蛋白浓度。用放线菌酮 (CH) 和红霉素 (ERY) 抑制蛋白质合成表明，内源性和 picloram 刺激的蛋白质合成是 80S 细胞质核糖体的功能，而不是 70S 叶绿体或线粒体核糖体的功能。