Lipid Profiling of 20 Mammalian Cells by Capillary Microsampling Combined with High-Resolution Spectral Stitching Nanoelectrospray Ionization Direct-Infusion Mass Spectrometry,Analytical Chemistry

当前位置： X-MOL 学术 › Anal. Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Lipid Profiling of 20 Mammalian Cells by Capillary Microsampling Combined with High-Resolution Spectral Stitching Nanoelectrospray Ionization Direct-Infusion Mass Spectrometry
Analytical Chemistry ( IF 6.7 ) Pub Date : 2021-07-16 , DOI: 10.1021/acs.analchem.1c00373
Tianrun Xu _{1,

2} , Hang Li ₁ , Disheng Feng _{1,

2} , Peng Dou ₁ , Xianzhe Shi ₁ , Chunxiu Hu ₁ , Guowang Xu _{1,

2}

Affiliation

Studies of cellular metabolism can provide profound insights into the underlying molecular mechanisms and metabolic function. To date, the majority of cellular metabolism studies based on chromatography–mass spectrometry (MS) require population cells to obtain informative metabolome. These methods are not only time-consuming but also not suitable for amount-limited cell samples such as circulating tumor cells, stem cells, and neurons. Therefore, it is extremely essential to develop analytical methods enabling to detect metabolome from tens of cells in a high-throughput and high-sensitivity way. In this work, a novel platform for rapid and sensitive detection of lipidome in 20 mammalian cells was proposed using capillary microsampling combined with high-resolution spectral stitching nanoelectrospray ionization direct-infusion MS. It can be used to collect cells rapidly and accurately via the capillary microprobe, extract lipids directly in a 96-well plate using a spray solvent, and detect more than 500 lipids covering 19 lipid subclasses within 3 min. This novel platform was successfully applied to study the lipid features of different cancer cell types and subtypes as well as target cells from tissue samples. This study provides a strategy for determining the lipid species with rich information in tens of cells and demonstrates great potential for clinical applications.

中文翻译：

通过毛细管微量采样结合高分辨率光谱拼接纳米电喷雾电离直接注入质谱法对 20 个哺乳动物细胞进行脂质分析

细胞代谢的研究可以提供对潜在分子机制和代谢功能的深刻见解。迄今为止，大多数基于色谱-质谱 (MS) 的细胞代谢研究都需要群体细胞来获得信息代谢组。这些方法不仅耗时而且不适合循环肿瘤细胞、干细胞和神经元等数量有限的细胞样本。因此，开发能够以高通量和高灵敏度的方式检测来自数十个细胞的代谢组的分析方法是极其必要的。在这项工作中，提出了一种使用毛细管微量采样结合高分辨率光谱拼接纳米电喷雾电离直接输注 MS 快速灵敏检测 20 种哺乳动物细胞脂质组的新平台。它可用于通过毛细管微探针快速准确地收集细胞，使用喷雾溶剂直接在 96 孔板中提取脂质，并在 3 分钟内检测覆盖 19 个脂质亚类的 500 多种脂质。这种新型平台已成功应用于研究不同癌细胞类型和亚型以及来自组织样本的靶细胞的脂质特征。该研究提供了一种在数十个细胞中确定具有丰富信息的脂质种类的策略，并展示了巨大的临床应用潜力。这种新型平台已成功应用于研究不同癌细胞类型和亚型以及来自组织样本的靶细胞的脂质特征。该研究提供了一种在数十个细胞中确定具有丰富信息的脂质种类的策略，并展示了巨大的临床应用潜力。这种新型平台已成功应用于研究不同癌细胞类型和亚型以及来自组织样本的靶细胞的脂质特征。该研究提供了一种在数十个细胞中确定具有丰富信息的脂质种类的策略，并展示了巨大的临床应用潜力。

更新日期：2021-07-27

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南