微藻利用包裹碳酸酐酶的浮珠直接空气捕获 CO2,ACS Sustainable Chemistry & Engineering

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微藻利用包裹碳酸酐酶的浮珠直接空气捕获 CO2
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2021-07-15 , DOI: 10.1021/acssuschemeng.1c01618
Xiaoyin Xu _{1,

2} , Sandra E. Kentish ₁ , Gregory J. O. Martin ₂

Affiliation

微藻培养有望成为大气碳的 CO ₂汇，以及作为食物和化学原料的可持续来源。然而，由于大气 CO ₂的扩散太慢，目前大规模微藻培养受到需要从点源提供二氧化碳的限制。碳酸酐酶 (CA) 是一种促进大气 CO ₂溶解的有效酶这可用于增强这种温室气体的光合作用吸收。在这里，我们研究了一种将 CA 保留在藻类池塘表面的方法，以便在封装成浮力海藻酸钙珠之前通过将 CA 与戊二醛 (GA) 交联来促进直接空气捕获。考马斯蓝染色和 Wilbur-Anderson 分析证实了 CA 与珠子的成功结合。显微图像显示石蜡包埋的藻酸盐框架。CA-GA 磁珠在 10 个检测循环中几乎保留了所有的水解酶活性。与 22.7 ± 0.5 mg L ^–1天^–1的自然增长率相比，游离 CA 和 CA-GA 珠将盐生小球藻的生产力提高到 37 ± 3 mg L ^–1天^–1和 40 ± 1 mg L ^–1天^–1分别。CA-GA 珠进一步为三轮微藻培养提供了稳定的生长增强，证实这些漂浮珠可以很容易地回收和重复使用，这对于工业生物质生产很有前景。

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Direct Air Capture of CO2 by Microalgae with Buoyant Beads Encapsulating Carbonic Anhydrase

Microalgae cultures have promise as a CO₂ sink for atmospheric carbon and as a sustainable source of food and chemical feedstocks. However, large-scale microalgae cultivation is currently limited by the need to provide carbon dioxide from point sources, as the diffusion of atmospheric CO₂ is too slow. Carbonic anhydrase (CA) is an effective enzyme to facilitate the dissolution of atmospheric CO₂ that could be used to enhance the photosynthetic uptake of this greenhouse gas. Here we investigate a means of retaining CA at the surface of algae ponds to facilitate direct air capture by cross-linking CA with glutaraldehyde (GA) before encapsulation into buoyant calcium alginate beads. Coomassie Blue dyeing and Wilbur–Anderson assays confirmed the successful bonding of CA to the beads. Microscopic images showed the paraffin-embedded alginate framework. The CA–GA beads retain virtually all hydrase activity throughout 10 assay cycles. Compared with a natural growth rate of 22.7 ± 0.5 mg L^–1 day^–1, free CA and CA–GA beads increased the productivity of Nannochloropsis salina to 37 ± 3 mg L^–1 day^–1 and 40 ± 1 mg L^–1 day^–1, respectively. The CA–GA beads further provided a stable growth enhancement for three rounds of microalgae cultivation, confirming that these buoyant beads can be readily recovered and re-used, which is promising for industrial biomass production.

更新日期：2021-07-26

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