Abstract Vitamin E succinate was synthesized in organic solvents using a modified Novozym-435 as catalyst. In order to improve the catalytic performance of Novozym-435, the enzyme was modified using acetic anhydride, propionic anhydride and succinic anhydride separately. We found that both the hydrolytic activity and the thermal stability of the modified Novozym-435 were enhanced compared with the unmodified enzyme. The modified Novozym-435 catalysts were used to synthesize the succinate derivative of vitamin E. Compared with the native Novozym-435, the catalytic activity of the modified novozym-435 in promoting the synthesis of vitamin E succinate was dramatically increased, with the novozym-435 modified with succinic anhydride (N435-S) as the most active catalyst. Conditions for the synthesis of vitamin E succinate were also optimized. A mixture of tert -butanol and DMSO (volume ratio of 2 : 3) was the most suitable medium for the reaction, whereas the appropriate molar ratio of vitamin E to succinic anhydride and reaction temperature were 1 : 5 and 40 (C, respectively. Under these reaction conditions, the yield of vitamin E succinate reached 94.4%. N435-S could be reused for five batches.

中文翻译：

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使用化学修饰的 Novozym-435 酶催化合成维生素 E 琥珀酸酯

摘要 以改性Novozym-435为催化剂，在有机溶剂中合成了维生素E琥珀酸酯。为了提高Novozym-435的催化性能，分别使用乙酸酐、丙酸酐和丁二酸酐对该酶进行了修饰。我们发现与未修饰的酶相比，修饰后的 Novozym-435 的水解活性和热稳定性都得到了增强。改性Novozym-435催化剂用于合成维生素E琥珀酸酯衍生物。与天然Novozym-435相比，改性novozym-435促进维生素E琥珀酸酯合成的催化活性显着提高，novozym- 435 以琥珀酸酐 (N435-S) 为活性最高的催化剂进行改性。维生素E琥珀酸酯的合成条件也进行了优化。