Facile Fabrication of Electrospun Nanofiber Membrane-Integrated PDMS Microfluidic Chip via Silver Nanowires-Uncured PDMS Adhesive Layer,ACS Macro Letters

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Facile Fabrication of Electrospun Nanofiber Membrane-Integrated PDMS Microfluidic Chip via Silver Nanowires-Uncured PDMS Adhesive Layer
ACS Macro Letters ( IF 5.1 ) Pub Date : 2021-07-12 , DOI: 10.1021/acsmacrolett.1c00256
Junyeol Rhyou ₁ , Jaeseung Youn ₁ , Seongsu Eom ₁ , Dong Sung Kim _{1,

2,

3}

Affiliation

Although direct electrospinning has been frequently utilized to develop a nanofiber membrane-integrated microfluidic chip, the dielectric substrate material retards the deposition of electrospun nanofibers on the substrate, and the rough surface formed by deposited nanofibers hinders the successful sealing. In this study we introduce a facile fabrication process of an electrospun nanofiber membrane-integrated polydimethylsiloxane (PDMS) microfluidic chip, called a NFM-PDMS chip, by applying the functional layer. The functional layer consists of a silver nanowires (AgNWs)-embedded uncured PDMS adhesive layer (SNUP), which not only effectively concentrates the electric field toward the PDMS substrate, but also provides a smooth surface for robust sealing. The AgNWs in the SNUP play a crucial role as a grounded collector and enable approximately 4× faster electrospinning than the conventional method, forming a free-standing nanofiber membrane. The uncured PDMS adhesive layer in the SNUP maintains the smooth surface after electrospinning and allows the rapid and leakage-free bonding of the NFM-PDMS chip using plasma treatment. A practical application of the NFM-PDMS chip is demonstrated by culturing the human keratinocyte cell line, HaCaT cells. The HaCaT cells are well grown on the free-standing nanofiber membrane under dynamic flow conditions, maintaining good viability over 95% for 7 days of culture.

中文翻译：

通过银纳米线-未固化的 PDMS 粘合剂层轻松制造电纺纳米纤维膜集成的 PDMS 微流控芯片

尽管直接静电纺丝经常被用于开发纳米纤维膜集成的微流控芯片，但介电基底材料会阻碍静电纺丝纳米纤维在基底上的沉积，并且由沉积的纳米纤维形成的粗糙表面阻碍了成功的密封。在这项研究中，我们通过应用功能层，介绍了一种名为 NFM-PDMS 芯片的电纺纳米纤维膜集成聚二甲基硅氧烷 (PDMS) 微流控芯片的简便制造工艺。功能层由嵌入银纳米线 (AgNWs) 的未固化 PDMS 粘合剂层 (SNUP) 组成，它不仅可以有效地将电场集中到 PDMS 基板上，而且还可以提供光滑的表面以实现牢固的密封。SNUP 中的 AgNW 作为接地收集器发挥着至关重要的作用，使静电纺丝的速度比传统方法快约 4 倍，从而形成独立的纳米纤维膜。SNUP 中未固化的 PDMS 粘合剂层在静电纺丝后保持光滑的表面，并允许使用等离子体处理快速、无泄漏地粘合 NFM-PDMS 芯片。NFM-PDMS 芯片的实际应用是通过培养人类角质形成细胞系 HaCaT 细胞来证明的。HaCaT 细胞在动态流动条件下在独立的纳米纤维膜上生长良好，培养 7 天时保持良好的存活率超过 95%。SNUP 中未固化的 PDMS 粘合剂层在静电纺丝后保持光滑的表面，并允许使用等离子体处理快速、无泄漏地粘合 NFM-PDMS 芯片。NFM-PDMS 芯片的实际应用是通过培养人类角质形成细胞系 HaCaT 细胞来证明的。HaCaT 细胞在动态流动条件下在独立的纳米纤维膜上生长良好，培养 7 天时保持良好的存活率超过 95%。SNUP 中未固化的 PDMS 粘合剂层在静电纺丝后保持光滑的表面，并允许使用等离子体处理快速、无泄漏地粘合 NFM-PDMS 芯片。NFM-PDMS 芯片的实际应用是通过培养人类角质形成细胞系 HaCaT 细胞来证明的。HaCaT 细胞在动态流动条件下在独立的纳米纤维膜上生长良好，培养 7 天时保持良好的存活率超过 95%。

更新日期：2021-07-20

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