<br>与商业肥料相比，揭示大豆植物对氢氧化铜纳米线的反应的代谢和蛋白质组特征,Environmental Science & Technology

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与商业肥料相比，揭示大豆植物对氢氧化铜纳米线的反应的代谢和蛋白质组特征
Environmental Science & Technology ( IF 10.8 ) Pub Date : 2021-07-09 , DOI: 10.1021/acs.est.1c00839
Sanghamitra Majumdar _{1,

2} , Randall W Long ₃ , Jay S Kirkwood ₄ , Anastasiia S Minakova ₁ , Arturo A Keller _{1,

2}

Affiliation

对铜基纳米材料与作物相互作用的机理理解对于探索其在精准农业中的应用及其对植物健康的影响至关重要。我们研究了大豆 ( Glycine max ) 植物在实际暴露浓度下对叶面施用氢氧化铜纳米线 (CNW) 的生物反应。使用商业铜基杀菌剂 (Kocide)、溶解的铜离子和未经处理的对照进行比较，以确定生理、细胞和分子水平上的独特特征。暴露于 CNW（0.36、1.8 和 9 mg CNW/植物）32 天后，与 Kocide（10 μg/g）相比，新发育的组织积累了显着高水平的 Cu（18-60 μg/g）；然而，与其他铜处理相比，铜从 CNW 处理部位转移到其他组织的速度较慢。与 Kocide 一样，中剂量和高剂量的 CNW 暴露改变了组织中 Co、Mn、Zn 和 Fe 的积累，并增强了光合作用活性。对 CNW 处理的大豆植物叶子的蛋白质组学和代谢组学分析表明，存在剂量依赖性反应，导致主要生物过程的激活，包括光合作用、能量产生、脂肪酸代谢、木质素生物合成和碳水化合物代谢。与 CNW 治疗相反，Kocide 暴露导致氧化应激反应增加和氨基酸代谢激活。

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Unraveling Metabolic and Proteomic Features in Soybean Plants in Response to Copper Hydroxide Nanowires Compared to a Commercial Fertilizer

Mechanistic understanding of the interaction of copper-based nanomaterials with crops is crucial for exploring their application in precision agriculture and their implications on plant health. We investigated the biological response of soybean (Glycine max) plants to the foliar application of copper hydroxide nanowires (CNWs) at realistic exposure concentrations. A commercial copper based-fungicide (Kocide), dissolved copper ions, and untreated controls were used for comparison to identify unique features at physiological, cellular, and molecular levels. After 32 d of exposure to CNW (0.36, 1.8, and 9 mg CNW/plant), the newly developed tissues accumulated significantly high levels of Cu (18–60 μg/g) compared to Kocide (10 μg/g); however, the rate of Cu translocation from the site of CNW treatment to other tissues was slower compared to other Cu treatments. Like Kocide, CNW exposure at medium and high doses altered Co, Mn, Zn, and Fe accumulation in the tissues and enhanced photosynthetic activities. The proteomic and metabolomic analyses of leaves from CNW-treated soybean plants suggest a dose-dependent response, resulting in the activation of major biological processes, including photosynthesis, energy production, fatty acid metabolism, lignin biosynthesis, and carbohydrate metabolism. In contrast to CNW treatments, Kocide exposure resulted in increased oxidative stress response and amino acid metabolism activation.

更新日期：2021-07-09

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