Impact of feed concentration on milk protein fractionation by hollow fiber microfiltration membranes in diafiltration mode,Separation and Purification Technology

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Impact of feed concentration on milk protein fractionation by hollow fiber microfiltration membranes in diafiltration mode
Separation and Purification Technology ( IF 8.1 ) Pub Date : 2021-07-09 , DOI: 10.1016/j.seppur.2021.119278
Roland Schopf ₁ , Ulrich Kulozik ₁

Affiliation

Microfiltration (0.1 µm) in the diafiltration mode is performed using hollow fiber membranes(HFM) to separate the casein micelles and whey proteins in skim milk at 10 °C. This method of using HFM has not been widely applied in dairy technology. HFM differ from more established module systems such as ceramic tubular membranes(CTM) and spiral-wound membranes(SWM) in their geometry, flow conditions, and propensity to deposit formation. The objective of this study is to investigate the efficiency of fractionation according to the pre-concentration before starting diafiltration when using ultrafiltration permeate as diafiltration medium for transferring the whey proteins into the permeate. The concentration having constant pressure drop is compared with that having constant feed volume flow. The optimal process conditions are determined based on the following criteria: flux, whey protein transmission, whey protein mass flow, and time required for one volume turnover, i.e., diafiltration step. The process is found to be optimum at a transmembrane pressure of 0.5 bar, constant pressure drop of 1.0 bar m−1, and concentration factor(CF) of 2.5. At a CF of 3, 80% whey protein depletion was achieved after 2.5 diafiltration steps. Therefore, HFM are confirmed as effective alternatives to SWM and CTM and are optimized in terms of pre-concentration of the casein fraction before starting diafiltration.

中文翻译：

饲料浓度对渗滤模式下中空纤维微滤膜牛奶蛋白分离的影响

使用中空纤维膜（HFM）在渗滤模式下进行微滤（0.1 μm），以在 10 °C 下分离脱脂牛奶中的酪蛋白胶束和乳清蛋白。这种使用 HFM 的方法尚未在乳品技术中得到广泛应用。HFM 与陶瓷管状膜（CTM）和螺旋缠绕膜（SWM）等更成熟的模块系统在几何形状、流动条件和沉积物形成倾向方面有所不同。本研究的目的是研究当使用超滤渗透液作为渗滤介质将乳清蛋白转移到渗透液中时，在开始渗滤前根据预浓缩进行分馏的效率。将具有恒定压降的浓度与具有恒定进料体积流量的浓度进行比较。最佳工艺条件根据以下标准确定：通量、乳清蛋白传输、乳清蛋白质量流量和一个体积周转所需的时间，即渗滤步骤。发现该工艺在 0.5 bar 的跨膜压力、1.0 bar m-1 的恒定压降和 2.5 的浓度因子（CF）下最佳。在 CF 为 3 时，经过 2.5 个洗滤步骤后，乳清蛋白去除率达到 80%。因此，HFM 被证实是 SWM 和 CTM 的有效替代品，并在开始渗滤之前对酪蛋白组分的预浓缩进行了优化。

更新日期：2021-07-09

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