Real-time Tracking and Sensing of Cu+ and Cu2+ with a Single SERS Probe in the Live Brain: Toward Understanding Why Copper Ions Were Increased upon Ischemia,Angewandte Chemie International Edition

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Real-time Tracking and Sensing of Cu+ and Cu2+ with a Single SERS Probe in the Live Brain: Toward Understanding Why Copper Ions Were Increased upon Ischemia
Angewandte Chemie International Edition ( IF 16.1 ) Pub Date : 2021-07-06 , DOI: 10.1002/anie.202106193
Jiaqi Liu ₁ , Zhichao Liu ₁ , Weikang Wang ₁ , Yang Tian ₁

Affiliation

The imbalance of Cu⁺ and Cu²⁺ in the brain is closely related to neurodegenerative diseases. However, it still lacks of effective analytical methods for simultaneously determining the concentrations of Cu⁺ and Cu²⁺. Herein, we created a novel SERS probe (CuSP) to real-time track and accurately quantify extracellular concentrations of Cu⁺ and Cu²⁺ in the live brain. The present CuSP probe demonstrated specific ability for recognition of Cu⁺ and Cu²⁺ in a dual-recognition mode. Then, a microarray consisting of 8 CuSP probes with high tempo-spatial resolution and good accuracy was constructed for tracking and simultaneously biosensing of Cu⁺ and Cu²⁺ in the cerebral cortex of living brain. Using our powerful tool, it was found that that the concentrations of Cu²⁺ and Cu⁺ were increased by ≈4.26 and ≈1.80 times upon ischemia, respectively. Three routes were first discovered for understanding the mechanisms of the increased concentrations of Cu⁺ and Cu²⁺ during ischemia.

中文翻译：

在活脑中使用单个 SERS 探针实时跟踪和感应 Cu+ 和 Cu2+：了解为什么缺血时铜离子增加

大脑中Cu ⁺和Cu ²⁺的失衡与神经退行性疾病密切相关。然而，目前还缺乏同时测定Cu ⁺和Cu ²⁺浓度的有效分析方法。在此，我们创建了一种新型 SERS 探针 (CuSP) 来实时跟踪和准确量化活脑中Cu ⁺和 Cu ^{2+ 的}细胞外浓度。本CuSP探针表现出识别Cu ⁺和Cu ^{2+ 的}特异性能力双识别模式。然后，构建了一个由 8 个具有高时空分辨率和良好精度的 CuSP 探针组成的微阵列，用于跟踪和同时生物传感活体大脑皮层中的 Cu ⁺和 Cu ²⁺。使用我们强大的工具，发现缺血时Cu ²⁺和Cu ⁺的浓度分别增加≈4.26 和≈1.80 倍。首次发现了三种途径，用于了解缺血期间Cu ⁺和Cu ²⁺浓度增加的机制。

更新日期：2021-09-14

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