小麦籽粒发育过程中蛋白质和代谢物的空间分布及其在灌浆过程中的差异调节反应,The Plant Journal

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小麦籽粒发育过程中蛋白质和代谢物的空间分布及其在灌浆过程中的差异调节反应
The Plant Journal ( IF 6.2 ) Pub Date : 2021-07-06 , DOI: 10.1111/tpj.15410
Shuang Zhang ₁ , Arindam Ghatak ₁ , Mitra Mohammadi Bazargani ₂ , Prasad Bajaj ₃ , Rajeev K Varshney _{3,

4} , Palak Chaturvedi ₁ , Dong Jiang ₅ , Wolfram Weckwerth _{1,

6}

Affiliation

籽粒灌浆和籽粒发育是植物生命周期中必不可少的生物学过程，最终有助于所有谷类作物的最终种子产量和质量。不同小麦 ( Triticum aestivum ) 的研究L.) 有助于种子整体发育的谷物成分非常稀缺。我们对小麦籽粒的四种不同发育成分（种皮、胚、胚乳和腔液）进行了蛋白质组学和代谢组学分析，以表征早期和晚期谷物发育过程中的分子过程。花后 12、15、20 和 26 天的凝胶内鸟枪蛋白质组学分析 (DAA) 揭示了 15 484 种已鉴定和定量的蛋白质，其中种皮中鉴定出 410 种差异表达蛋白，胚胎中鉴定出 815 种，胚胎中鉴定出 372 种。胚乳和 492 腔内的液体。大量选定的候选蛋白质揭示了与发育和籽粒灌浆相关的空间和时间分辨蛋白质功能。参与淀粉合成的多种小麦蛋白异构体，例如蔗糖合酶，淀粉磷酸化酶、颗粒结合和可溶性淀粉合酶、丙酮酸磷酸二激酶、14-3-3 蛋白以及糖前体在小麦籽粒发育过程中的丰度发生了重大的组织依赖性变化，这表明淀粉生物合成控制之间存在密切的相互作用。蛋白质二硫键异构酶家族的不同异构体以及谷氨酰胺水平都参与了麦谷蛋白大分子聚合物的模式，显示出不同的空间和时间丰度，揭示了它们作为小麦面筋质量指标的特定作用。归类为细胞生长/分裂和蛋白质合成/降解功能类别的蛋白质在早期阶段（12 和 15 DAA）更为丰富。在代谢组水平上，所有组织，尤其是腔液显示出高度不同的代谢物谱。

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Spatial distribution of proteins and metabolites in developing wheat grain and their differential regulatory response during the grain filling process

Grain filling and grain development are essential biological processes in the plant’s life cycle, eventually contributing to the final seed yield and quality in all cereal crops. Studies of how the different wheat (Triticum aestivum L.) grain components contribute to the overall development of the seed are very scarce. We performed a proteomics and metabolomics analysis in four different developing components of the wheat grain (seed coat, embryo, endosperm, and cavity fluid) to characterize molecular processes during early and late grain development. In-gel shotgun proteomics analysis at 12, 15, 20, and 26 days after anthesis (DAA) revealed 15 484 identified and quantified proteins, out of which 410 differentially expressed proteins were identified in the seed coat, 815 in the embryo, 372 in the endosperm, and 492 in the cavity fluid. The abundance of selected protein candidates revealed spatially and temporally resolved protein functions associated with development and grain filling. Multiple wheat protein isoforms involved in starch synthesis such as sucrose synthases, starch phosphorylase, granule-bound and soluble starch synthase, pyruvate phosphate dikinase, 14-3-3 proteins as well as sugar precursors undergo a major tissue-dependent change in abundance during wheat grain development suggesting an intimate interplay of starch biosynthesis control. Different isoforms of the protein disulfide isomerase family as well as glutamine levels, both involved in the glutenin macropolymer pattern, showed distinct spatial and temporal abundance, revealing their specific role as indicators of wheat gluten quality. Proteins binned into the functional category of cell growth/division and protein synthesis/degradation were more abundant in the early stages (12 and 15 DAA). At the metabolome level all tissues and especially the cavity fluid showed highly distinct metabolite profiles. The tissue-specific data are integrated with biochemical networks to generate a comprehensive map of molecular processes during grain filling and developmental processes.

更新日期：2021-08-30

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