Polian vesicle is originally regarded as a hematopoietic and inflammatory response organ in sea cucumber by the operations of cell depletion and heterogeneous cells injection, respectively. In the present study, to reveal the role and immune mechanisms of polian vesicle in response to pathogen, Vibrio splendidus, we first performed a comparative transcriptome analysis for the cells from polian vesicle wall in V. splendidus-challenged Apostichopus japonicus through RNA high-throughput sequencing technology. Briefly, 465,356,848 clean reads were obtained after cleaning up low-quality reads in total. Approximately 73% of the sequenced reads could be aligned to the reference genome of A. japonicus. The DEGs of CG (control group) vs TG 24 h (24 h post-infection group), CG vs TG 72 h (72 h post-infection group) and TG 24 h vs TG 72 h were 3762, 1391 and 3258, respectively. Gene Ontology (GO) annotation assay revealed that those genes associated with the processes such as cell process, cell, binding and catalytic activity were significantly induced in all three groups post V. splendidus infection. KEGG enrichment analysis suggested the DEGs in TG 24 h were enriched in Toll-like receptor (TLR) signaling pathway, complement and coagulation cascades, antigen processing and presentation and IL-17 signaling pathway compared with that in CG, while the pathways including ribosome biogenesis in eukaryotes, DNA replication, and cell cycle related with cell proliferation were mainly enriched in TG 72 h than that of CG. Furthermore, six important DEGs were chosen and showed the consistent expression patterns with the results of RNA-seq by qPCR. Overall, our analysis towards the current data demonstrates that polian vesicle may play an essential role in the regulation of immune response in A. japonicus and provide new insights into hematopoietic function of polian vesicle in response to pathogen infection.

Polian囊泡最初被认为是海参中的造血和炎症反应器官，分别通过细胞耗竭和异质细胞注射的操作。在本研究中，为了揭示 Polian 囊泡对病原体Vibrio splendidus的作用和免疫机制，我们首先通过 RNA 高通量对V. splendidus攻击的刺参的Polian 囊泡壁细胞进行了转录组比较分析测序技术。简而言之，在清除低质量读取后，总共获得了 465,356,848 个干净读取。大约 73% 的测序读数可以与A. japonicus的参考基因组比对. CG（对照组）vs TG 24 h（感染后24 h组）、CG vs TG 72 h（感染后72 h组）和TG 24 h vs TG 72 h的DEG分别为3762、1391和3258 . 基因本体论（GO）注释分析显示，与细胞过程、细胞、结合和催化活性等过程相关的基因在所有三组中均被显着诱导。感染。KEGG富集分析表明，与CG相比，TG 24 h中的DEGs在Toll样受体（TLR）信号通路、补体和凝血级联、抗原加工和呈递以及IL-17信号通路中富集，而包括核糖体生物发生在内的通路在真核生物中，与细胞增殖相关的 DNA 复制和细胞周期主要在 TG 中富集 72 h，而不是 CG。此外，选择了六个重要的 DEG，并通过 qPCR 显示出与 RNA-seq 结果一致的表达模式。总体而言，我们对当前数据的分析表明，Polian 囊泡可能在A. japonicus的免疫反应调节中发挥重要作用 并提供对 polian 囊泡响应病原体感染的造血功能的新见解。