使用组织来源的肾小球基底膜的肾脏过滤屏障的仿生体外模型,Advanced Healthcare Materials

当前位置： X-MOL 学术 › Adv. Healthcare Mater. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

使用组织来源的肾小球基底膜的肾脏过滤屏障的仿生体外模型
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2021-07-03 , DOI: 10.1002/adhm.202002275
Dan Wang ₁ , Snehal Sant ₁ , Nicholas Ferrell _{1,

2,

3}

Affiliation

肾小球滤过屏障 (GFB) 过滤血液以去除毒素，同时保留循环中的高分子量蛋白质。肾小球基底膜 (GBM) 和足细胞是高度特化的上皮细胞，是过滤屏障的关键组成部分。GBM 充当分子进入滤液的物理屏障。足细胞粘附在 GBM 的滤液侧，并进一步限制高分子量分子进入滤液。在这里，开发了肾小球滤过屏障的 3D 细胞培养模型，以评估 GBM 和足细胞在介导分子扩散中的作用。GBM 是从哺乳动物肾脏中分离出来的，以概括体内基底膜的组成和力学。GFB 模型表现出与体内过滤屏障相当的分子选择性。单独的 GBM 为白蛋白和 Ficoll 的通过提供了严格的屏障。足细胞进一步限制分子扩散。使用次氯酸模拟糖尿病肾病典型的 GBM 损伤，并导致分子扩散增加。该系统可以作为一个平台来评估 GBM 损伤、足细胞损伤的影响，以及改变的足细胞-GBM 相互作用对肾脏微血管通透性的相互影响。

"点击查看英文标题和摘要"

A Biomimetic In Vitro Model of the Kidney Filtration Barrier Using Tissue-Derived Glomerular Basement Membrane

The glomerular filtration barrier (GFB) filters the blood to remove toxins while retaining high molecular weight proteins in the circulation. The glomerular basement membrane (GBM) and podocytes, highly specialized epithelial cells, are critical components of the filtration barrier. The GBM serves as a physical barrier to passage of molecules into the filtrate. Podocytes adhere to the filtrate side of the GBM and further restrict passage of high molecular weight molecules into the filtrate. Here, a 3D cell culture model of the glomerular filtration barrier to evaluate the role of the GBM and podocytes in mediating molecular diffusion is developed. GBM is isolated from mammalian kidneys to recapitulate the composition and mechanics of the in vivo basement membrane. The GFB model exhibits molecular selectivity that is comparable to the in vivo filtration barrier. The GBM alone provides a stringent barrier to passage of albumin and Ficoll. Podocytes further restrict molecular diffusion. Damage to the GBM that is typical of diabetic kidney disease is simulated using hypochlorous acid and results in increased molecular diffusion. This system can serve as a platform to evaluate the effects of GBM damage, podocyte injury, and reciprocal effects of altered podocyte–GBM interactions on kidney microvascular permeability.

更新日期：2021-08-17

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南