Vanilla is the most popular aroma among consumers and the authentication of its main compound – vanillin – remains a current challenge in transformed products, whose low vanillin concentration is a limitation to SNIF-NMR use. A new and fast NMR-isotopomics approach is proposed with modified INEPT ¹³C NMR pulse sequence implemented for vanillin intramolecular composition determination. Position-specific isotopic compositions were estimated through statistical modelling from incomplete experimental data (since quaternary carbons did not benefit from polarization transfer), allowing the comparison of databases analyzed either with single pulse or multipulse method. The new approach was validated: a sensitivity gain was achieved while preserving performances similar to reference method. Vanillin ex-C₄-sugars, a growing source in the market, was described and blends with biosynthesized vanillin were positively discriminated from vanillin ex-beans, the main adulteration target. The method was applied on vanilla sugars to demonstrate its applicability for convenience good authentication. Vanillin quantity required for analysis was reduced to 50 mg, opening path to new control possibilities in finished food products.

香草是最受消费者欢迎的香气，其主要化合物香草醛的认证仍然是转化产品的当前挑战，其低香草醛浓度限制了 SNIF-NMR 的使用。提出了一种新的、快速的 NMR-同位素组学方法，其中改进了 INEPT ¹³ C NMR 脉冲序列，用于香草醛分子内成分测定。位置特定的同位素组成是通过从不完整的实验数据（因为季碳没有从极化转移中受益）的统计建模来估计的，从而可以比较使用单脉冲或多脉冲方法分析的数据库。新方法得到验证：在保持与参考方法相似的性能的同时实现了灵敏度增益。香兰素 ex-C ₄描述了市场上不断增长的糖源，并且与生物合成香兰素的混合物与主要掺假目标香兰素前豆类有明显区别。该方法应用于香草糖以证明其适用于方便良好的认证。分析所需的香兰素量减少到 50 mg，为成品食品中的新控制可能性开辟了道路。