Abstract Polycyclic aromatic hydrocarbons (PAHs) are a group of persistent organic compounds. White rot fungi (WRF) have attracted much attention due to their strong capabilities for the initial transformation of PAHs. To better understand the metabolic pathway of PAHS by WRF in the presence of ligninolytic enzymes and cytochrome P450 monooxygenase (P450s), degradation by a high laccase-producing fungus, Pycnoporus sanguineus 14, was examined in detail. This strain with high laccase activity (2516.7 U L−1) exhibited 45.6% biodegradation of phenanthrene (PHE) and 90.1% of benz[a]anthracene (BAA) under in vivo conditions. Gas chromatography–mass spectrometry (GC-MS) was used to identify 2-methylphenol as the product of PHE, and benz[a]anthracene-7,12-dione, 2,3-dihydroxy-3-phenylpropanoic acid and citramalic acid were identified as products of BAA. The results of an in vitro experiment indicated that the strain could initially transform PHE to 2-dibenzofuranol by intracellular enzymes containing P450s, or 9,10-phenanthrenedione by extracellular laccase, and could initially transform BAA to benz[a]anthracene-7,12-dione via extracellular laccase.

中文翻译：

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白腐菌 Pycnoporus sanguineus 14 中菲和苯并[a]蒽代谢途径的多样性

摘要 多环芳烃（PAHs）是一组持久性有机化合物。白腐真菌（WRF）因其对多环芳烃初始转化的强大能力而备受关注。为了更好地了解在木质素分解酶和细胞色素 P450 单加氧酶 (P450) 存在下 WRF 对 PAHS 的代谢途径，详细检查了高产漆酶真菌 Pycnoporus sanguineus 14 的降解。这种具有高漆酶活性 (2516.7 U L-1) 的菌株在体内条件下表现出 45.6% 的菲 (PHE) 和 90.1% 的苯并 [a] 蒽 (BAA) 的生物降解。气相色谱-质谱联用仪 (GC-MS) 用于鉴定作为 PHE 产物的 2-甲基苯酚，苯并[a]蒽-7,12-二酮、2,3-二羟基-3-苯基丙酸和柠檬酸被认定为 BAA 的产品。