In this paper, a simple, rapid, and low-cost colorimetric method was designed based on Co/Fe-MOFs-iodide composite for the quantitative detection of H₂S. It is know that iodide can improve the catalytic activity of bimetallic porous material Co/Fe-MOFs via adsorption into the framework of MOFs. Herein, we demonstrate a novel strategy to enhance the peroxidase-like activity of MOFs. Compared to horseradish peroxidase (HRP), the kinetic measurement results show that Co/Fe-MOFs-iodide exhibits excellent affinity to substrates, promoting electron transfer. Due to the synergetic effect of Co/Fe-MOFs and iodide, and rapid electron transfer process, Co/Fe-MOFs-iodide demonstrates improved peroxidase-like activity. As a proof-of-concept application, a novel, highly sensitive H₂S colorimetric method is established with a detection limit (LOD) of 0.33 nM. In the absence of iodide, LOD is approx. 200-fold higher than that of the amplified colorimetric assay. The proposed method can also accurately detect traces of H₂S in serum samples.

本文设计了一种基于Co/Fe-MOFs-碘化物复合物的简单、快速、低成本的比色法定量检测H ₂ S。已知碘化物可以提高双金属多孔材料的催化活性Co/Fe-MOFs 通过吸附到 MOFs 的框架中。在此，我们展示了一种增强 MOF 类过氧化物酶活性的新策略。与辣根过氧化物酶 (HRP) 相比，动力学测量结果表明 Co/Fe-MOFs-碘化物对底物具有优异的亲和力，促进电子转移。由于 Co/Fe-MOFs 和碘化物的协同作用以及快速的电子转移过程，Co/Fe-MOFs-碘化物表现出改善的类过氧化物酶活性。作为概念验证应用，一种新颖的、高度敏感的 H ₂S 比色法的检测限 (LOD) 为 0.33 nM。在没有碘化物的情况下，LOD 约为。比扩增比色法高 200 倍。所提出的方法还可以准确地检测血清样品中的痕量H ₂ S。