Salvia plebeia R. Br. polysaccharides (SPP) against RSV (respiratory syncytial virus) infection: Antiviral effect and mechanisms of action,Biomedicine & Pharmacotherapy

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Salvia plebeia R. Br. polysaccharides (SPP) against RSV (respiratory syncytial virus) infection: Antiviral effect and mechanisms of action
Biomedicine & Pharmacotherapy ( IF 6.9 ) Pub Date : 2021-06-24 , DOI: 10.1016/j.biopha.2021.111843
Yi-Yu Liang ₁ , Kun-Wei Li ₁ , Feng-Jv Niu ₂ , Ying Li ₁ , Hao-Cheng Wei ₁ , Yun-Lei Dai ₁ , Yun-Yu Wang ₁ , Chang-Zheng Zhou ₁ , Xin-Huan Wan ₁

Affiliation

To investigate the antiviral effect of R. Br. polysaccharides (SPP) against RSV and underlying mechanisms. SPP was extracted alcohol-precipitation method and extract was separated into various fractions using ultrafiltration method. The polysaccharide content was determined using UV-Vis. Antiviral effect of SPP and fractions was measured using MTT method and Reed-Muench method. Sixty Balb/c mice were randomly divided into 6 groups, and received either Ribavirin or SPP. Their body weight and food intake were recorded every day throughout the experiment period. The lung index inhibition ratio and pulmonary virus titer were determined followed by the histological analysis of lungs. Furthermore, time-of-addition and effective stage analysis were carried out to determine the mechanism of action. The TLR-3 and TLR-4 levels in the lungs were determined using qRT-PCR. The levels of IFN-γ, IL-2 and TNF-α in serum were determined using ELISA. The SPP content is 4.396%. SPP has shown a good anti-RSV effect both (TI = 123.041) and models. The antiviral activity of fractions with molecular weight ≥ 10,000 is found to possess more potent antiviral activity than other fractions. SPP inhibits the RSV proliferation and reduces the lung lesions induced by RSV. The mechanism of action involves the inhibition of TLR-3 and TLR-4 in lungs, up-regulation of IFN-γ and IL-2, and down-regulation of TNF-α in serum. It is also shown to improve the body’s immune function. SPP has a potential to treat diseases caused by RSV.

中文翻译：

鼠尾草 (Salvia plebeia R. Br.)多糖（SPP）抗RSV（呼吸道合胞病毒）感染：抗病毒作用和作用机制

研究 R. Br 的抗病毒作用。多糖 (SPP) 对抗 RSV 及其潜在机制。 SPP采用醇沉法提取，并采用超滤法将提取物分离成各个组分。使用UV-Vis测定多糖含量。采用MTT法和Reed-Muench法测定SPP及其级分的抗病毒作用。 60 只 Balb/c 小鼠被随机分为 6 组，并接受利巴韦林或 SPP 治疗。在整个实验期间每天记录他们的体重和食物摄入量。测定肺指数抑制率和肺部病毒滴度，然后对肺进行组织学分析。此外，还进行了添加时间和有效阶段分析以确定作用机制。使用 qRT-PCR 测定肺部 TLR-3 和 TLR-4 水平。采用ELISA法测定血清中IFN-γ、IL-2和TNF-α的水平。 SPP含量为4.396%。 SPP (TI = 123.041) 和模型都显示出良好的抗 RSV 效果。发现分子量 ≥ 10,000 的级分的抗病毒活性比其他级分具有更强的抗病毒活性。 SPP抑制RSV增殖并减轻RSV引起的肺部损伤。作用机制涉及抑制肺部TLR-3和TLR-4，上调IFN-γ和IL-2，下调血清中TNF-α。它还被证明可以提高人体的免疫功能。 SPP 有潜力治疗 RSV 引起的疾病。

更新日期：2021-06-24

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