Using an antibody raised against jackbean pro-asparaginyl endopeptidase and a substrate for jackbean asparaginyl endopeptidase, immunoblot analysis and quantitation of the activity were performed for extracts from cotyledons of both developing and germinating seeds of five legume species, jackbean, soybean, Vigna mungo, peanut and kidney bean. The immunoblot analysis indicated the occurrence of multiple polypeptides immunologically related to jackbean asparaginyl endopeptidase in each legume examined. Most of the polypeptides detected in developing and dry seeds had different molecular masses from those in germinating seeds. Asparaginyl endopeptidase activity as measured by using dinitrophenyl(DNP)-Pro-Glu-Ala-Asn-NH2 as a substrate was detected in extracts from developing and germinating legume seeds tested. A possible role of this asparaginyl endopeptidase activity during seed germination is discussed.

中文翻译：

---

豆类种子发育和萌发中的天冬酰胺内肽酶：免疫学检测和定量

使用针对 jackbean pro-asparaginyl 内肽酶产生的抗体和 jackbean asparaginyl endopeptidase 的底物，对来自五种豆科植物、jackbean、大豆、Vigna mungo、花生的发育和发芽种子的子叶提取物进行了免疫印迹分析和活性定量分析和芸豆。免疫印迹分析表明在每个检查的豆科植物中出现了多个与长豆角天冬酰胺内肽酶免疫相关的多肽。在发育和干燥种子中检测到的大多数多肽与发芽种子中的分子质量不同。通过使用二硝基苯基 (DNP)-Pro-Glu-Ala-Asn-NH2 作为底物测量的天冬酰胺酰内肽酶活性在来自测试的发育和发芽豆类种子的提取物中检测到。