Peroxynitrite (ONOO⁻) plays important roles in many pathophysiological processes and its subcellular detection draws increasing attention. In this study, we designed and prepared a novel lysosome-targetable fluorescent probe (E)-2-(benzo[d]thiazol-2- yl)-4-methyl-6-((morpholinoimino)methyl)phenol (BMP) for selective detection of ONOO⁻ in living systems by incorporating a reactive morpholino hydrazone as new ONOO⁻ response site into a benzothiazole derivative as fluorophore. After reaction with ONOO⁻, an obvious fluorescence increase (83-fold) was observed accompanied with distinct dual colorimetric and fluorescence changes. Probe BMP displayed the merits of fast response (<3 s), ultrasensitivity (LOD = 6 nM) and high selectivity towards ONOO⁻ over other physiological species including ROS/RNS. Most importantly, the probe was capable of imaging ONOO⁻ in lysosomes of living cells with good cell permeation and negligible cytotoxicity. Therefore, this research provides an effective tool to study the functions of ONOO⁻ in lysosomes.

过氧亚硝酸盐（ONOO ^-）在许多病理生理过程中起着重要作用，其亚细胞检测引起越来越多的关注。在本研究中，我们设计并制备了一种新型溶酶体靶向荧光探针 ( E )-2-(benzo[d]thiazol-2-yl)-4-methyl-6-((morpholinoimino)methyl)phenol ( BMP ) ONOO的选择性检测^-在通过将反应性吗啉代腙作为新ONOO生命系统^-响应现场成苯并噻唑衍生物如荧光团。与ONOO ^-反应后，观察到明显的荧光增加（83 倍），伴随着明显的比色和荧光双重变化。探针BMP显示响应速度快（<3秒），ultrasensitivity（LOD = 6 1nM）和高选择性朝ONOO的优点^-在其他生理物种，包括ROS / RNS。最重要的是，探测器有能力ONOO成像^-在生活与良好的细胞渗透和可以忽略不计的细胞毒性细胞的溶酶体。因此，这项研究提供了一个有效的工具来研究ONOO的功能^-在溶酶体中。