A magnetic β-cyclodextrin (MCD) surface molecularly imprinted polymer (MIP) based on deep eutectic solvents (DESs) as cross-linker and functional monomer (MCD@DES-MIP) was successfully synthesized for the specific recognition of bovine hemoglobin (BHb). The adsorption behavior of MCD@DES-MIP for BHb was investigated by adsorption thermodynamics, adsorption kinetics, and pH control experiments. The maximum adsorption capacity of MCD@DES-MIP for BHb under the optimized conditions was 195.94 mg g⁻¹ and the imprinting factor was 4.68. In addition, the competitive adsorption experiments demonstrated that MCD@DES-MIP showed excellent selective extraction ability for BHb in the binary mixture of BHb and bovine serum albumin (BSA). The actual sample analysis manifested that MCD@DES-MIP effectively separated BHb from complex samples. The results of circular dichroism spectra proved that the secondary structure of BHb did not change during elution. The result indicated that MCD@DES-MIP can be used as a new imprinting material for the separation and purification of BHb.

Graphical abstract

基于深共熔溶剂（DESs）作为交联剂和功能单体（MCD@DES-MIP）的磁性β-环糊精（MCD）表面分子印迹聚合物（MIP）被成功合成用于特异性识别牛血红蛋白（BHb） . 通过吸附热力学、吸附动力学和 pH 控制实验研究了 MCD@DES-MIP 对 BHb 的吸附行为。优化条件下MCD@DES-MIP对BHb的最大吸附量为195.94 mg g ^-1印记因子为4.68。此外，竞争吸附实验表明，MCD@DES-MIP 对 BHb 和牛血清白蛋白 (BSA) 的二元混合物中的 BHb 显示出优异的选择性提取能力。实际样品分析表明，MCD@DES-MIP 有效地将 BHb 从复杂样品中分离出来。圆二色光谱结果证明BHb的二级结构在洗脱过程中没有发生变化。结果表明，MCD@DES-MIP可作为一种新的印迹材料用于BHb的分离纯化。

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