Owing to lack of the effective chromogenic groups, a convenient, precise, and real-time assay for the detection of methyl mercaptan (CH₃SH) still remains many huge challenges, but it is of great importance to the food and agribusiness related industries. In this work, we proposed an interesting and efficient fluorescence sensing strategy-based alcohol oxidase (AOX)-triggered in situ synthesis of bovine serum albumin-stabilized copper nanoclusters (BSA-CuNCs) for sensitive and selective detection of CH₃SH. In the presence of CH₃SH, AOX-assisted O₂ could catalyze it to be H₂O₂, which could further trigger BSA-Cu²⁺ system to in situ synthesize of BSA-CuNCs, emitting fluorescence signals. On this basis, CH₃SH concentration-dependent fluorescence emission of BSA-CuNCs was observed, thus allowing for developing a novel fluorescence sensing platform for reporting the levels of CH₃SH with a broad linear range from 1.0 to 300.0 μM and a low detection limit of 0.19 μM. More significantly, we exploited this sensing strategy to monitor CH₃SH in real ham samples with acceptable results. This work gives a new insight into applying in situ synthesis strategy to develop a new category of fluorescence sensing platform, achieving convenient, low-cost, reliable, and precise measurements in real food and agriculture-related systems, thereby manifesting a promising potential in food safety monitoring.

由于缺乏有效的显色基团，一种方便、精确、实时的甲硫醇（CH ₃ SH）检测方法仍然面临着许多巨大的挑战，但它对食品和农业相关行业具有重要意义。在这项工作中，我们提出了一种有趣且有效的荧光传感策略，基于乙醇氧化酶 (AOX) 触发原位合成牛血清白蛋白稳定的铜纳米簇 (BSA-CuNCs)，用于灵敏和选择性地检测 CH ₃ SH。在 CH ₃ SH存在下，AOX 辅助的 O ₂可以将其催化为 H ₂ O ₂，这可以进一步触发 BSA-Cu ²⁺系统原位合成 BSA-CuNCs，发出荧光信号。在此基础上，观察到 BSA-CuNCs 的CH ₃ SH 浓度依赖性荧光发射，从而允许开发一种新型荧光传感平台，用于报告 CH ₃ SH水平，线性范围为 1.0 至 300.0 μM，检测率低限制为 0.19 μM。更重要的是，我们利用这种传感策略来监测真实火腿样品中的CH ₃ SH，并获得可接受的结果。这项工作为原位应用提供了新的见解 合成策略开发新型荧光传感平台，在真实的食品和农业相关系统中实现方便、低成本、可靠和精确的测量，从而在食品安全监测中展现出广阔的潜力。