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Mechanism of Lentinan Intestinal Absorption: Clathrin-Mediated Endocytosis and Macropinocytosis
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2021-06-16 , DOI: 10.1021/acs.jafc.1c00349 Ziming Zheng 1, 2 , Xianglin Pan 1 , Haoyu Wang 1 , Zhijing Wu 1 , Mitchell A Sullivan 3 , Yuxuan Liu 1 , Junxi Liu 1 , Kaiping Wang 4 , Yu Zhang 1, 2
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2021-06-16 , DOI: 10.1021/acs.jafc.1c00349 Ziming Zheng 1, 2 , Xianglin Pan 1 , Haoyu Wang 1 , Zhijing Wu 1 , Mitchell A Sullivan 3 , Yuxuan Liu 1 , Junxi Liu 1 , Kaiping Wang 4 , Yu Zhang 1, 2
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Lentinan (LNT), a typical triple helix β-glucan extracted from Lentinus edodes, has been widely used as a functional food and an orally administered drug. However, its oral pharmacokinetics has been rarely reported. The aim of this work is to systematically study the pharmacokinetics and intestinal absorption mechanism of LNT after oral administration. Radioactive 99m-technetium (99mTc) was introduced to label LNT to determine the plasma concentration, tissue distribution, and excretion of the β-glucan in rats after oral administration. The results confirmed the absorption of LNT, with the maximal plasma concentration reached at 1 h. 5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein (DTAF) was used to label LNT to explore the absorption mechanism of LNT, utilizing both a Ussing chamber and a monolayer of Caco-2 cells. These transport assays showed that LNT could penetrate through the intestine and epithelial monolayer, which was mediated by macropinocytosis and clathrin-mediated endocytosis. These findings provide a pharmacokinetic reference for LNT and help provide a greater understanding of the absorption of β-glucans in general.
中文翻译：
香菇多糖肠道吸收机制：网格蛋白介导的内吞作用和巨胞饮作用
香菇多糖（LNT）是一种从香菇中提取的典型三螺旋β-葡聚糖，已被广泛用作功能性食品和口服药物。但其口服药代动力学鲜见报道。本工作的目的是系统研究LNT口服后的药代动力学和肠道吸收机制。放射性 99m-锝 ( 99m引入 Tc) 标记 LNT，以确定大鼠口服后 β-葡聚糖的血浆浓度、组织分布和排泄。结果证实了LNT的吸收，最大血浆浓度在1小时达到。5-([4,6-Dichlorotriazin-2-yl]amino) 荧光素 (DTAF) 用于标记 LNT，以利用 Ussing 室和单层 Caco-2 细胞探索 LNT 的吸收机制。这些转运试验表明，LNT 可以穿透肠道和上皮单层，这是由巨胞饮作用和网格蛋白介导的内吞作用介导的。这些发现为 LNT 提供了药代动力学参考，并有助于更好地了解 β-葡聚糖的一般吸收。
更新日期：2021-07-07
中文翻译：
香菇多糖肠道吸收机制：网格蛋白介导的内吞作用和巨胞饮作用
香菇多糖（LNT）是一种从香菇中提取的典型三螺旋β-葡聚糖，已被广泛用作功能性食品和口服药物。但其口服药代动力学鲜见报道。本工作的目的是系统研究LNT口服后的药代动力学和肠道吸收机制。放射性 99m-锝 ( 99m引入 Tc) 标记 LNT，以确定大鼠口服后 β-葡聚糖的血浆浓度、组织分布和排泄。结果证实了LNT的吸收，最大血浆浓度在1小时达到。5-([4,6-Dichlorotriazin-2-yl]amino) 荧光素 (DTAF) 用于标记 LNT，以利用 Ussing 室和单层 Caco-2 细胞探索 LNT 的吸收机制。这些转运试验表明，LNT 可以穿透肠道和上皮单层，这是由巨胞饮作用和网格蛋白介导的内吞作用介导的。这些发现为 LNT 提供了药代动力学参考，并有助于更好地了解 β-葡聚糖的一般吸收。
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