An “Off–On” Rhodamine 6G Hydrazide-Based Output Platform for Fluorescence and Visual Dual-Mode Detection of Lead(II),Journal of Agricultural and Food Chemistry

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An “Off–On” Rhodamine 6G Hydrazide-Based Output Platform for Fluorescence and Visual Dual-Mode Detection of Lead(II)
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2021-06-16 , DOI: 10.1021/acs.jafc.1c02568
Xiaoqian Xie _{1,

2} , Mingfei Pan _{1,

2} , Liping Hong _{1,

2} , Kaixin Liu _{1,

2} , Jingying Yang _{1,

2} , Shan Wang _{1,

2} , Shuo Wang _{1,

2}

Affiliation

In this study, the rhodamine 6G hydrazide (R6GH) complex was synthesized to develop an “off–on” output platform for fluorescence and visual dual-mode analysis of lead(II) (Pb²⁺). The prepared R6GH complex using the heat to reflux reaction of rhodamine 6G (R6G) and hydrazine hydrate was characterized through FT-IR, MS, ¹H NMR, and ¹³C NMR and demonstrated to have good fluorescence stability and reversibility. The microenvironment for Pb²⁺ detection has been optimized in detail. Under the optimal conditions, the “off–on” R6GH-based fluorescence output platform showed a good response to Pb²⁺ in the concentration range of 0.05–6.0 μM (R² = 0.9851) with a limit of detection (LOD) of 0.02 μM. Furthermore, at three spiked Pb²⁺ levels in the selected agricultural (tap water, soil) and food (fish, shrimp) samples, the developed R6GH-based fluorescence assays obtained a significant recovery range of 84.0–102.0% (RSD < 5.0%, n = 3), which had a good correlation with the results from ICP-MS (R² = 0.9915). The developed R6GH immobilized paper-based array sensor can reach the lower LOD (2.5 μM) for Pb²⁺ through the naked eye. By combining with LAB analysis, a good linear response was obtained in the Pb²⁺ concentration range of 1.0–50.0 μM. These results indicated that the developed R6GH probe had great application potential in accurate detection of fluorescence and rapid visual and semiquantitative screening for Pb²⁺.

中文翻译：

用于铅 (II) 荧光和视觉双模式检测的“关-开”罗丹明 6G 酰肼输出平台

在本研究中，合成了罗丹明 6G 酰肼 (R6GH) 络合物，以开发用于铅 (II) (Pb ²⁺ ) 荧光和视觉双模式分析的“关闭-开启”输出平台。利用罗丹明6G（R6G）与水合肼加热回流反应制备的R6GH配合物通过FT-IR、MS、 ¹ H NMR、 ¹³ C NMR进行表征，证明具有良好的荧光稳定性和可逆性。 Pb ²⁺检测的微环境进行了详细优化。在最佳条件下，基于R6GH的“关-开”荧光输出平台在0.05-6.0 μM浓度范围内（ R ² = 0.9851）对Pb ²⁺表现出良好的响应，检测限（LOD）为0.02微米。此外，在选定的农业（自来水、土壤）和食品（鱼、虾）样品中的三种 Pb ²⁺浓度下，开发的基于 R6GH 的荧光测定法获得了 84.0–102.0% 的显着回收率范围（RSD < 5.0%）， n = 3），与 ICP-MS 的结果具有良好的相关性（ R ² = 0.9915）。开发的R6GH固定纸基阵列传感器可以通过肉眼达到Pb ²⁺的较低LOD（2.5 μM）。将g与LAB分析相结合，在Pb ²⁺浓度1.0–50.0 μM范围内获得了良好的线性响应。这些结果表明，所开发的R6GH探针在荧光的精确检测以及Pb ²⁺的快速目视半定量筛查方面具有巨大的应用潜力。

更新日期：2021-06-30

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