Tenascin-C 促进鼻咽癌的上皮间质转化和 mTOR 信号通路。,Oncology Letters

当前位置： X-MOL 学术 › Oncol. Lett. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Tenascin-C 促进鼻咽癌的上皮间质转化和 mTOR 信号通路。
Oncology Letters ( IF 2.5 ) Pub Date : 2021-05-29 , DOI: 10.3892/ol.2021.12831
Xiang Cheng ₁ , Fen Li ₁ , Zezhang Tao ₁

Affiliation

Tenascin-C (TNC) 是一种大的细胞外基质糖蛋白，可促进细胞粘附和组织重塑，并参与细胞信号通路的转导。本研究旨在研究 TNC 的作用并确定其在鼻咽癌 (NPC) 中的作用。使用NPC数据集和NPC组织的免疫组织化学分析来分析TNC基因的转录和表达。进行加权基因共表达网络和基因富集分析，以确定 TNC 对 NPC 影响的潜在分子机制。TNC 表达在 NPC 细胞中受到抑制，其影响在体外和体内均已确定. 结果表明，与正常组织相比，NPC 组织中的 TNC 基因转录和表达较高。值得注意的是，TNC 敲低抑制了 NPC 细胞增殖、迁移和侵袭。此外，TNC 敲低抑制了小鼠的肿瘤生长。在体外，敲低 TNC 抑制上皮间质转化 (EMT) 并降低 NPC 细胞中 PI3K/AKT/mTOR 信号通路的活性。总之，这些结果表明 TNC 促进 NPC 细胞中的细胞增殖、EMT 和 PI3K/AKT/mTOR 信号通路的活性，从而起到致癌基因的作用。

"点击查看英文标题和摘要"

Tenascin-C promotes epithelial-to-mesenchymal transition and the mTOR signaling pathway in nasopharyngeal carcinoma.

Tenascin-C (TNC) is a large extracellular matrix glycoprotein that promotes cell adhesion and tissue remodeling, and is involved in the transduction of cellular signaling pathways. The present study aimed to investigate the role of TNC and determine its effect in nasopharyngeal carcinoma (NPC). TNC gene transcription and expression were analyzed using the NPC dataset and immunohistochemistry analysis of NPC tissues. Weighted gene co-expression network and gene enrichment analyses were performed to determine the potential molecular mechanisms underlying the effects of TNC in NPC. TNC expression was suppressed in NPC cells, and the effects were determined both in vitro and in vivo. The results demonstrated that TNC gene transcription and expression were high in NPC tissues compared with normal tissues. Notably, TNC knockdown inhibited NPC cell proliferation, migration and invasion. In addition, TNC knockdown inhibited tumor growth in mice. In vitro, TNC knockdown inhibited epithelial-to-mesenchymal transition (EMT) and decreased activity of the PI3K/AKT/mTOR signaling pathway in NPC cells. Taken together, these results suggest that TNC promotes cell proliferation, EMT and activity of the PI3K/AKT/mTOR signaling pathway in NPC cells, and thus functions as an oncogene.

更新日期：2021-06-16

点击分享查看原文

点击收藏

公开下载

阅读更多本刊新发论文本刊介绍/投稿指南