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Liposomal Controlled Release Ag-Activated DNAzyme Cycle Amplification on a 2D Pyrene COF-Based Photocathode for α-Synuclein Immunosensing
Analytical Chemistry ( IF 6.7 ) Pub Date : 2021-06-11 , DOI: 10.1021/acs.analchem.1c01789 Yao Gao 1 , Jinling Zhang 1 , Xuechen Zhang 1 , Jiawen Li 1 , Rui Zhang 1 , Wenbo Song 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2021-06-11 , DOI: 10.1021/acs.analchem.1c01789 Yao Gao 1 , Jinling Zhang 1 , Xuechen Zhang 1 , Jiawen Li 1 , Rui Zhang 1 , Wenbo Song 1
Affiliation
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Ultrasensitive and accurate monitoring of ultralow-level biomarkers is imperiously needed in clinical diagnosis. So far, exploring high-performance photocathodes and developing new sensing strategies have remained central challenges in photoelectrochemical bioassays. Herein, a two-dimensional (2D) pyrene covalent organic framework (COF, PAF-130) is exemplified for the first time as a high-performance photocathode for precise immunosensing of α-synuclein (α-Syn) by integrating a DNAzyme-induced signal cycle amplification strategy with Ag nanoparticles (NPs)-mediated liposomal immunoassay. Through sequential immunobinding, lysis treatment, and acidolysis, numerous Ag+ ions are released, and then they activate the DNAzyme, which further recycles the cleavage of hairpin DNA (HDNA) on the photoelectrode and induces signal cycle amplification. As a result, an ultralow detection limit (3.6 fg/mL) and a wide linear range (10–5–103 ng/mL) are achieved, which surpass those of most methods reported so far. The proposed sensing approach can be readily extended to detect various biomarkers by substituting the biorecognition events, providing great promise for biomedical and related applications.
中文翻译：
在基于 2D 芘 COF 的光电阴极上进行脂质体控释 Ag 激活 DNA 酶循环放大，用于 α-突触核蛋白免疫传感
临床诊断迫切需要超低水平生物标志物的超灵敏和准确监测。迄今为止，探索高性能光电阴极和开发新的传感策略仍然是光电化学生物测定的核心挑战。在此，首次将二维 (2D) 芘共价有机框架 (COF, PAF-130) 作为高性能光电阴极，通过整合 DNAzyme 诱导的 α-突触核蛋白 (α-Syn) 进行精确免疫传感。使用银纳米粒子 (NPs) 介导的脂质体免疫测定的信号循环放大策略。通过顺序免疫结合、裂解处理和酸解，大量的 Ag +离子被释放，然后它们激活 DNAzyme，进一步回收光电极上的发夹 DNA (HDNA) 裂解并诱导信号循环放大。因此，实现了超低检测限 (3.6 fg/mL) 和宽线性范围 (10 –5 –10 3 ng/mL)，超过了迄今为止报道的大多数方法。通过替代生物识别事件，所提出的传感方法可以很容易地扩展到检测各种生物标志物，为生物医学和相关应用提供了广阔的前景。
更新日期：2021-06-22
中文翻译：
在基于 2D 芘 COF 的光电阴极上进行脂质体控释 Ag 激活 DNA 酶循环放大，用于 α-突触核蛋白免疫传感
临床诊断迫切需要超低水平生物标志物的超灵敏和准确监测。迄今为止，探索高性能光电阴极和开发新的传感策略仍然是光电化学生物测定的核心挑战。在此，首次将二维 (2D) 芘共价有机框架 (COF, PAF-130) 作为高性能光电阴极，通过整合 DNAzyme 诱导的 α-突触核蛋白 (α-Syn) 进行精确免疫传感。使用银纳米粒子 (NPs) 介导的脂质体免疫测定的信号循环放大策略。通过顺序免疫结合、裂解处理和酸解，大量的 Ag +离子被释放，然后它们激活 DNAzyme，进一步回收光电极上的发夹 DNA (HDNA) 裂解并诱导信号循环放大。因此，实现了超低检测限 (3.6 fg/mL) 和宽线性范围 (10 –5 –10 3 ng/mL)，超过了迄今为止报道的大多数方法。通过替代生物识别事件，所提出的传感方法可以很容易地扩展到检测各种生物标志物，为生物医学和相关应用提供了广阔的前景。
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