胶原蛋白和 EPS 成分对铜绿假单胞菌生物膜粘弹性的影响,Soft Matter

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胶原蛋白和 EPS 成分对铜绿假单胞菌生物膜粘弹性的影响
Soft Matter ( IF 2.9 ) Pub Date : 2021-6-4 , DOI: 10.1039/d1sm00463h
Minhaz Ur Rahman ₁ , Derek F Fleming ₂ , Indranil Sinha ₁ , Kendra P Rumbaugh ₂ , Vernita D Gordon ₃ , Gordon F Christopher ₁

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铜绿假单胞菌是一种机会性病原体，每年导致数千人死亡，部分原因是它能够形成由嵌入自我分泌的细胞外多糖 (EPS)、e-DNA 和蛋白质基质中的细菌组成的生物膜。在慢性伤口中，生物膜暴露于宿主细胞外基质，其中胶原蛋白是主要成分。细菌 EPS 如何与宿主胶原相互作用以及这种相互作用是否影响生物膜粘弹性尚不清楚。由于生物膜的物理破坏通常用于去除生物膜，因此了解胶原蛋白对生物膜粘弹性的影响可能会启用新的治疗策略，更好地适应宿主环境中生长的生物膜。在这项工作中，生物膜在模拟体内条件的不同浓度胶原蛋白的存在下生长。为了探索胶原蛋白与 EPS 的相互作用，使用具有不同 EPS 生产模式的9 株铜绿假单胞菌菌株来生长生物膜。粒子追踪微流变学用于表征生物膜在两天内的机械发育。研究发现，无论系统中存在 EPS，胶原蛋白都会降低生物膜顺应性并增加相对弹性。然而，当生物膜过度产生 EPS 时，这种影响就会最小化。通过与细菌结合或物理缠结，胶原蛋白似乎成为EPS事实上的组成部分。

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Effect of collagen and EPS components on the viscoelasticity of Pseudomonas aeruginosa biofilms

Pseudomonas aeruginosa is an opportunistic pathogen that causes thousands of deaths every year in part due to its ability to form biofilms composed of bacteria embedded in a matrix of self-secreted extracellular polysaccharides (EPS), e-DNA, and proteins. In chronic wounds, biofilms are exposed to the host extracellular matrix, of which collagen is a major component. How bacterial EPS interacts with host collagen and whether this interaction affects biofilm viscoelasticity is not well understood. Since physical disruption of biofilms is often used in their removal, knowledge of collagen's effects on biofilm viscoelasticity may enable new treatment strategies that are better tuned to biofilms growing in host environments. In this work, biofilms are grown in the presence of different concentrations of collagen that mimic in vivo conditions. In order to explore collagen's interaction with EPS, nine strains of P. aeruginosa with different patterns of EPS production were used to grow biofilms. Particle tracking microrheology was used to characterize the mechanical development of biofilms over two days. Collagen is found to decrease biofilm compliance and increase relative elasticity regardless of the EPS present in the system. However, this effect is minimized when biofilms overproduce EPS. Collagen appears to become a de facto component of the EPS, through binding to bacteria or physical entanglement.

更新日期：2021-06-10

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