abaI/abaR 群体感应系统影响鲍曼不动杆菌的致病性,Frontiers in Microbiology

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abaI/abaR 群体感应系统影响鲍曼不动杆菌的致病性
Frontiers in Microbiology ( IF 4.0 ) Pub Date : 2021-06-08 , DOI: 10.3389/fmicb.2021.679241
Xiaoyu Sun ₁ , Zhaohui Ni ₁ , Jie Tang ₁ , Yue Ding ₁ , Xinlei Wang ₂ , Fan Li _{1,

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Affiliation

鲍曼不动杆菌是一种革兰氏阴性病原体，已成为全球卫生保健机构最棘手的病原体之一。细菌群体感应 (QS) 是一种细胞间通讯过程，它依赖于自诱导剂 (AI) 信号的产生、分泌和检测，以共享有关细胞密度的信息并相应地调节基因表达。鲍曼不动杆菌毒力的分子和遗传基础仍然知之甚少。因此，详细研究了abaI/abaR群体感应系统对鲍曼不动杆菌生长特性、形态、生物膜形成、抗性、运动性和毒力的贡献。RNA-seq 分析表明，涉及能量产生和转化的各个方面的基因，缬氨酸，亮氨酸和异亮氨酸降解以及脂质转运和代谢与细菌致病性有关。我们的工作提供了对 abaI/abaR 群体感应系统影响鲍曼不动杆菌致病性的新见解。我们建议靶向 AHL 合酶 abaI 可以提供一种有效的毒力减弱策略。相反，阻断自诱导剂合酶 - 受体 abaR 会引起不可预测的后果，这可能会导致细菌毒力增强。

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The abaI/abaR quorum sensing system effects pathogenicity in Acinetobacter baumannii

Acinetobacter baumannii is a Gram-negative pathogen that has emerged as one of the most troublesome pathogens for health care institutions globally. Bacterial quorum sensing (QS) is a process of cell-to-cell communication that relies on the production, secretion and detection of autoinducer (AI) signals to share information about cell density and regulate gene expression accordingly. The molecular and genetic basis of Acinetobacter baumannii virulence remains poorly understood. Therefore, the contribution of the abaI/abaR quorum sensing system to growth characteristics, morphology, biofilm formation, resistance, motility and virulence of Acinetobacter baumannii was studied in detail. RNA-seq analysis indicated that genes involved in various aspects of energy production and conversion, Valine, leucine and isoleucine degradation and lipid transport and metabolism are associated with bacterial pathogenicity. Our work provides a new insight into abaI/abaR quorum sensing system effects pathogenicity in A. baumannii. We propose that targeting the AHL synthase enzyme abaI could provide an effective strategy for attenuating virulence. On the contrary, interdicting the autoinducer synthase–receptor abaR elicits unpredictable consequences, which may lead to enhanced bacterial virulence.

更新日期：2021-06-08

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