Many studies have demonstrated that cabbages possess various biological activities, and our previous studies confirmed that cyanidin-3-diglucoside-5-glucoside (CY3D5G), the major core of red cabbage anthocyanins, exhibited in vitro antioxidant activity. This study further investigated the protective effects of CY3D5G derivative from red cabbage juice (RCJ) on oxidative stress and lifespan in cells and Caenorhabditis elegans, green cabbage juice (GCJ) was used as control. RCJ rather than GCJ significantly improved cell viability and decreased lactate dehydrogenase release in H₂O₂-induced caco-2 cells. RCJ significantly increased survival during oxidative and heat stress and mean lifespan in C. elegans by 171.63% and 31.64%, and 28.16%, respectively, while GCJ treatment showed no significant effects (p < 0.05). These results might be attributed to significantly (p < 0.05) higher contents of total phenolics, ascorbic acid, glucosinolates, and anthocyanins in RCJ compared to those in GCJ. Additionally, both of them decreased autofluorescence and reproductive capacity, increased body length, but did not alter the intracellular ROS level. Prolonged lifespan by RCJ might require heat-shock transcription factor pathway, sirtuin signaling, and calmodulin kinase II pathway, independent of insulin/insulin-like growth factor-1 signaling pathway. RCJ showed promising antioxidant properties in caco-2 cells and C. elegans, which provided more information on the health benefits of cabbage.

中文翻译：

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红卷心菜而不是绿卷心菜可增加抗压性并延长秀丽隐杆线虫的寿命

许多研究表明，卷心菜具有多种生物活性，我们之前的研究证实，红甘蓝花青素的主要核心 cyanidin-3-diglucoside-5-glucoside (CY3D5G) 具有体外抗氧化活性。本研究进一步研究了来自红甘蓝汁 (RCJ) 的 CY3D5G 衍生物对细胞和秀丽隐杆线虫的氧化应激和寿命的保护作用，绿甘蓝汁 (GCJ) 用作对照。在 H ₂ O ₂诱导的 caco-2 细胞中，RCJ 而不是 GCJ 显着提高细胞活力并减少乳酸脱氢酶释放。RCJ 显着提高了秀丽隐杆线虫在氧化和热应激期间的存活率和平均寿命分别增加了 171.63% 和 31.64% 和 28.16%，而 GCJ 治疗没有显示出显着影响（p < 0.05）。这些结果可能归因于 ( p < 0.05) RCJ 中总酚类、抗坏血酸、硫代葡萄糖苷和花青素的含量显着高于 GCJ。此外，它们都降低了自发荧光和生殖能力，增加了体长，但没有改变细胞内 ROS 水平。RCJ 延长寿命可能需要热休克转录因子通路、sirtuin 信号通路和钙调蛋白激酶 II 通路，独立于胰岛素/胰岛素样生长因子-1 信号通路。RCJ 在 caco-2 细胞和秀丽隐杆线虫中显示出有希望的抗氧化特性，其中提供了有关卷心菜健康益处的更多信息。