Microchimica Acta ( IF 5.3 ) Pub Date : 2021-06-08 , DOI: 10.1007/s00604-021-04876-0
Mohamad Mahani 1 , Firouze Mahmoudi 2 , Javad Fassihi 3 , Zahra Hasani 4 , Faten Divsar 5
|
Diagnosis, treatment, and prediction of cancer progression require new targeting agents to specifically target cell surface receptors. Herein, we demonstrated fluorescent carbon quantum dots-molecularly imprinted polymer (CQD-MIP) for selective targeting and imaging of cancer cells. Carbon quantum dots (CQDs) were synthesized and characterized. The synthesized CQDs had average size of 1.5 nm and show intense fluorescence emission at wavelength of 450 nm with excitation at 370 nm. CQD-MIP nanoparticles imprinted with N-acetylneuraminic acid and glucuronic acid were prepared and characterized. CQD-MIPs were successfully applied for selective targeting and imaging of MCF-7, HepG-2, and NIH-3T3 cell lines. Non-imprinted polymer (NIP) showed no binding properties toward a target molecule. Non-imprinted polymer (NIP) and non-cancerous human cell lines were used for controlling the imprinting and targeting effects, respectively. Acceptable results were obtained with imprinted polymers on cancer cells.
Graphical abstract
中文翻译：
碳点嵌入的 N-乙酰神经氨酸和葡萄糖醛酸印迹聚合物用于癌细胞的靶向和成像
癌症进展的诊断、治疗和预测需要新的靶向剂来特异性靶向细胞表面受体。在此，我们展示了荧光碳量子点-分子印迹聚合物（CQD-MIP）用于癌细胞的选择性靶向和成像。合成并表征了碳量子点 (CQD)。合成的 CQD 的平均尺寸为 1.5 nm，在 450 nm 的波长下显示出强烈的荧光发射，在 370 nm 处激发。制备并表征了印有 N-乙酰神经氨酸和葡萄糖醛酸的 CQD-MIP 纳米颗粒。CQD-MIP 已成功应用于 MCF-7、HepG-2 和 NIH-3T3 细胞系的选择性靶向和成像。非印迹聚合物 (NIP) 对目标分子没有结合特性。非印迹聚合物 (NIP) 和非癌性人类细胞系分别用于控制印迹和靶向效应。在癌细胞上印迹聚合物获得了可接受的结果。
京公网安备 11010802027423号