Highly Sensitive Colorimetric/Surface-Enhanced Raman Spectroscopy Immunoassay Relying on a Metallic Core–Shell Au/Au Nanostar with Clenbuterol as a Target Analyte,Analytical Chemistry

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Highly Sensitive Colorimetric/Surface-Enhanced Raman Spectroscopy Immunoassay Relying on a Metallic Core–Shell Au/Au Nanostar with Clenbuterol as a Target Analyte
Analytical Chemistry ( IF 6.7 ) Pub Date : 2021-06-02 , DOI: 10.1021/acs.analchem.1c01487
Lihong Su ₁ , Huilan Hu ₁ , Yanli Tian ₁ , Conghui Jia ₁ , Lulu Wang ₁ , Han Zhang ₁ , Jianlong Wang ₁ , Daohong Zhang ₁

Affiliation

Lateral flow immunoassay (LFIA) has emerged as an effective technique in the field of food safety and environmental monitoring. However, sensitive and quantitative detection is still challenging for LFIAs in complex environments. In this work, a dual-model colorimetric/SERS lateral flow immunoassay for ultrasensitive determination of clenbuterol was constructed based on a metallic core–shell Au/Au nanostar acting as a multifunction tag. Raman reporter molecules are located between the core (AuNP) and shell (Au nanostar) to form a sandwich structure, which contributes to eliminate the environmental interference and improve the detection stability. In addition, the Au/Au nanostar provides a much higher Raman enhancement due to the presence of sharp tips and larger surface roughness in comparison with gold nanoparticles (AuNPs). Thus, on the basis of the antibody–antigen interaction, the dual-model immunoassay can produce strong colorimetric and surface-enhanced Raman spectroscopy (SERS) signals for highly sensitive detection of the target analyte, clenbuterol. Under optimal conditions, clenbuterol could be detected by the colorimetric model with a visual detection limit of 5 ng/mL. Meanwhile, the SERS signal of the Au/Au nanostar was accumulated on the test line for the SERS model detection with a quantitative detection limit as low as 0.05 ng/mL, which is at least 200-fold lower than that of the traditional AuNPs-based immunoassay. Furthermore, recovery rates of the proposed method in food samples were 86–110%. This dual-model immunoassay provides an effective tool for antibiotic residues analysis and demonstrates a broad potential for future applications in food safety monitoring.

中文翻译：

高灵敏度比色/表面增强拉曼光谱免疫分析依赖于金属核-壳 Au/Au Nanostar，以克仑特罗为目标分析物

横向流动免疫分析 (LFIA) 已成为食品安全和环境监测领域的有效技术。然而，对于复杂环境中的 LFIA 而言，灵敏和定量检测仍然具有挑战性。在这项工作中，基于金属核壳 Au/Au 纳米星作为多功能标签构建了双模型比色/SERS 侧流免疫测定法，用于超灵敏测定克仑特罗。拉曼报告分子位于核（AuNP）和壳（Au nanostar）之间形成夹心结构，有助于消除环境干扰，提高检测稳定性。此外，与金纳米粒子 (AuNPs) 相比，由于存在尖锐的尖端和更大的表面粗糙度，Au/Au 纳米星提供了更高的拉曼增强。因此，在抗体-抗原相互作用的基础上，双模型免疫测定可以产生强比色和表面增强拉曼光谱 (SERS) 信号，用于高灵敏度检测目标分析物克仑特罗。在最佳条件下，克仑特罗可以通过比色模型检测到，视觉检测限为 5 ng/mL。同时，Au/Au纳米星的SERS信号在SERS模型检测测试线上积累，定量检测限低至0.05 ng/mL，比传统AuNPs低至少200倍-基于免疫分析。此外，所提出的方法在食品样品中的回收率为 86-110%。

更新日期：2021-06-15

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